1eqk: Difference between revisions

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[[Image:1eqk.gif|left|200px]]
{{Seed}}
[[Image:1eqk.png|left|200px]]


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{{STRUCTURE_1eqk|  PDB=1eqk  |  SCENE=  }}  
{{STRUCTURE_1eqk|  PDB=1eqk  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF ORYZACYSTATIN-I, A CYSTEINE PROTEINASE INHIBITOR OF THE RICE, ORYZA SATIVA L. JAPONICA'''
===SOLUTION STRUCTURE OF ORYZACYSTATIN-I, A CYSTEINE PROTEINASE INHIBITOR OF THE RICE, ORYZA SATIVA L. JAPONICA===




==Overview==
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The three-dimensional structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica, has been determined in solution at pH 6.8 and 25 degrees C by (1)H and (15)N NMR spectroscopy. The main body (Glu13-Asp97) of oryzacystatin-I is well-defined and consists of an alpha-helix and a five-stranded antiparallel beta-sheet, while the N- and C-terminal regions (Ser2-Val12 and Ala98-Ala102) are less defined. The helix-sheet architechture of oryzacystatin-I is stabilized by a hydrophobic cluster formed between the alpha-helix and the beta-sheet and is considerably similar to that of monellin, a sweet-tasting protein from an African berry, as well as those of the animal cystatins studied, e.g., chicken egg white cystatin and human stefins A and B (also referred to as human cystatins A and B). Detailed structural comparison indicates that oryzacystatin-I is more similar to chicken cystatin, which belongs to the type-2 animal cystatins, than to human stefins A and B, which belong to the type-1 animal cystatins, despite different loop length.
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{{ABSTRACT_PUBMED_11101290}}


==About this Structure==
==About this Structure==
1EQK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa_japonica_group Oryza sativa japonica group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQK OCA].  
1EQK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa_japonica_group Oryza sativa japonica group]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQK OCA].  


==Reference==
==Reference==
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[[Category: Cystatin/monellin superfamily]]
[[Category: Cystatin/monellin superfamily]]
[[Category: Phytocystatin family]]
[[Category: Phytocystatin family]]
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Revision as of 22:43, 30 June 2008

File:1eqk.png

Template:STRUCTURE 1eqk

SOLUTION STRUCTURE OF ORYZACYSTATIN-I, A CYSTEINE PROTEINASE INHIBITOR OF THE RICE, ORYZA SATIVA L. JAPONICA

Template:ABSTRACT PUBMED 11101290

About this Structure

1EQK is a Single protein structure of sequence from Oryza sativa japonica group. Full experimental information is available from OCA.

Reference

Three-dimensional solution structure of oryzacystatin-I, a cysteine proteinase inhibitor of the rice, Oryza sativa L. japonica., Nagata K, Kudo N, Abe K, Arai S, Tanokura M, Biochemistry. 2000 Dec 5;39(48):14753-60. PMID:11101290

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