1fcv: Difference between revisions

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[[Image:1fcv.gif|left|200px]]
{{Seed}}
[[Image:1fcv.png|left|200px]]


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{{STRUCTURE_1fcv|  PDB=1fcv  |  SCENE=  }}  
{{STRUCTURE_1fcv|  PDB=1fcv  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF BEE VENOM HYALURONIDASE IN COMPLEX WITH HYALURONIC ACID TETRAMER'''
===CRYSTAL STRUCTURE OF BEE VENOM HYALURONIDASE IN COMPLEX WITH HYALURONIC ACID TETRAMER===




==Overview==
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BACKGROUND: Hyaluronic acid (HA) is the most abundant glycosaminoglycan of vertebrate extracellular spaces and is specifically degraded by a beta-1,4 glycosidase. Bee venom hyaluronidase (Hya) shares 30% sequence identity with human hyaluronidases, which are involved in fertilization and the turnover of HA. On the basis of sequence similarity, mammalian enzymes and Hya are assigned to glycosidase family 56 for which no structure has been reported yet. RESULTS: The crystal structure of recombinant (Baculovirus) Hya was determined at 1.6 A resolution. The overall topology resembles a classical (beta/alpha)(8) TIM barrel except that the barrel is composed of only seven strands. A long substrate binding groove extends across the C-terminal end of the barrel. Cocrystallization with a substrate analog revealed the presence of a HA tetramer bound to subsites -4 to -1 and distortion of the -1 sugar. CONCLUSIONS: The structure of the complex strongly suggest an acid-base catalytic mechanism, in which Glu113 acts as the proton donor and the N-acetyl group of the substrate is the nucleophile. The location of the catalytic residues shows striking similarity to bacterial chitinase which also operates via a substrate-assisted mechanism.
The line below this paragraph, {{ABSTRACT_PUBMED_11080624}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_11080624}}


==About this Structure==
==About this Structure==
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[[Category: Glycosidase family 56]]
[[Category: Glycosidase family 56]]
[[Category: Hyaluronic acid]]
[[Category: Hyaluronic acid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:03:48 2008''

Revision as of 00:03, 1 July 2008

File:1fcv.png

Template:STRUCTURE 1fcv

CRYSTAL STRUCTURE OF BEE VENOM HYALURONIDASE IN COMPLEX WITH HYALURONIC ACID TETRAMER

Template:ABSTRACT PUBMED 11080624

About this Structure

1FCV is a Single protein structure of sequence from Apis mellifera. Full crystallographic information is available from OCA.

Reference

Crystal structure of hyaluronidase, a major allergen of bee venom., Markovic-Housley Z, Miglierini G, Soldatova L, Rizkallah PJ, Muller U, Schirmer T, Structure. 2000 Oct 15;8(10):1025-35. PMID:11080624

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