1fd2: Difference between revisions

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[[Image:1fd2.gif|left|200px]]
{{Seed}}
[[Image:1fd2.png|left|200px]]


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{{STRUCTURE_1fd2|  PDB=1fd2  |  SCENE=  }}  
{{STRUCTURE_1fd2|  PDB=1fd2  |  SCENE=  }}  


'''SITE-DIRECTED MUTAGENESIS OF AZOTOBACTER VINELANDII FERREDOXIN I. (FE-S) CLUSTER-DRIVEN PROTEIN REARRANGEMENT'''
===SITE-DIRECTED MUTAGENESIS OF AZOTOBACTER VINELANDII FERREDOXIN I. (FE-S) CLUSTER-DRIVEN PROTEIN REARRANGEMENT===




==Overview==
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Azotobacter vinelandii ferredoxin I is a small protein that contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. Recently the x-ray crystal structure has been redetermined and the fdxA gene, which encodes the protein, has been cloned and sequenced. Here we report the site-directed mutation of Cys-20, which is a ligand of the [4Fe-4S] cluster in the native protein, to alanine and the characterization of the protein product by x-ray crystallographic and spectroscopic methods. The data show that the mutant protein again contains one [4Fe-4S] cluster and one [3Fe-4S] cluster. The new [4Fe-4S] cluster obtains its fourth ligand from Cys-24, a free cysteine in the native structure. The formation of this [4Fe-4S] cluster drives rearrangement of the protein structure.
The line below this paragraph, {{ABSTRACT_PUBMED_2153958}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_2153958}}


==About this Structure==
==About this Structure==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Stout, C D.]]
[[Category: Stout, C D.]]
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