|
|
| Line 1: |
Line 1: |
| [[Image:1fdz.gif|left|200px]] | | {{Seed}} |
| | [[Image:1fdz.png|left|200px]] |
|
| |
|
| <!-- | | <!-- |
| Line 9: |
Line 10: |
| {{STRUCTURE_1fdz| PDB=1fdz | SCENE= }} | | {{STRUCTURE_1fdz| PDB=1fdz | SCENE= }} |
|
| |
|
| '''N-ACETYLNEURAMINATE LYASE IN COMPLEX WITH PYRUVATE VIA BOROHYDRIDE REDUCTION'''
| | ===N-ACETYLNEURAMINATE LYASE IN COMPLEX WITH PYRUVATE VIA BOROHYDRIDE REDUCTION=== |
|
| |
|
|
| |
|
| ==Overview==
| | <!-- |
| We describe here a sub-family of enzymes related both structurally and functionally to N-acetylneuraminate lyase. Two members of this family (N-acetylneuraminate lyase and dihydrodipicolinate synthase) have known three-dimensional structures and we now proceed to show their structural and functional relationship to two further proteins, trans-o-hydroxybenzylidenepyruvate hydratase-aldolase and D-4-deoxy-5-oxoglucarate dehydratase. These enzymes are all thought to involve intermediate Schiff-base formation with their respective substrates. In order to understand the nature of this intermediate, we have determined the three-dimensional structure of N-acetylneuraminate lyase in complex with hydroxypyruvate (a product analogue) and in complex with one of its products (pyruvate). From these structures we deduce the presence of a closely similar Schiff-base forming motif in all members of the N-acetylneuraminate lyase sub-family. A fifth protein, MosA, is also confirmed to be a member of the sub-family although the involvement of an intermediate Schiff-base in its proposed reaction is unclear.
| | The line below this paragraph, {{ABSTRACT_PUBMED_9047371}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9047371 is the PubMed ID number. |
| | --> |
| | {{ABSTRACT_PUBMED_9047371}} |
|
| |
|
| ==About this Structure== | | ==About this Structure== |
| Line 35: |
Line 39: |
| [[Category: Lyase]] | | [[Category: Lyase]] |
| [[Category: Oxo-acid lyase]] | | [[Category: Oxo-acid lyase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:13:00 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:06:39 2008'' |
