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| [[Image:1fhy.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1fhy| PDB=1fhy | SCENE= }} | | {{STRUCTURE_1fhy| PDB=1fhy | SCENE= }} |
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| '''PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION'''
| | ===PSORALEN CROSS-LINKED D(CCGCTAGCGG) FORMS HOLLIDAY JUNCTION=== |
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| ==Overview==
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| The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11302703}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11302703 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11302703}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Holliday junction]] | | [[Category: Holliday junction]] |
| [[Category: Psoralen]] | | [[Category: Psoralen]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:20:48 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:16:29 2008'' |
