1fmp: Difference between revisions

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[[Image:1fmp.gif|left|200px]]
{{Seed}}
[[Image:1fmp.png|left|200px]]


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{{STRUCTURE_1fmp|  PDB=1fmp  |  SCENE=  }}  
{{STRUCTURE_1fmp|  PDB=1fmp  |  SCENE=  }}  


'''X-RAY ANALYSIS OF SUBSTRATE ANALOGS IN THE RICIN A CHAIN ACTIVE SITE'''
===X-RAY ANALYSIS OF SUBSTRATE ANALOGS IN THE RICIN A CHAIN ACTIVE SITE===




==Overview==
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Ricin A-chain is an N-glycosidase that hydrolyzes the adenine ring from a specific adenosine of rRNA. Formycin monophosphate (FMP) and adenyl(3'--&gt;5')guanosine (ApG) were bound to ricin A-chain and their structures elucidated by X-ray crystallography. The formycin ring stacks between tyrosines 80 and 123 and at least four hydrogen bonds are made to the adenine moiety. A residue invariant in this enzyme class, Arg180, appears to hydrogen bond to N-3 of the susceptible adenine. Three hypothetical models for binding a true hexanucleotide substrate, CGAGAG, are proposed. They incorporate adenine binding, shown by crystallography, but also include geometry likely to favor catalysis. For example, efforts have been made to orient the ribose ring in a way that allows solvent attack and oxycarbonium stabilization by the enzyme. The favored model is a simple perturbation of the tetraloop structure determined by nuclear magnetic resonance for similar polynucleotides. The model is attractive in that specific roles are defined for conserved protein residues. A mechanism of action is proposed. It invokes oxycarbonium ion stabilization on ribose by Glu177 in the transition state. Arg180 stabilizes anion development on the leaving adenine by protonation at N-3 and may activate a trapped water molecule that is the ultimate nucleophile in the depurination.
The line below this paragraph, {{ABSTRACT_PUBMED_1433290}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_1433290}}


==About this Structure==
==About this Structure==
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[[Category: Robertus, J D.]]
[[Category: Robertus, J D.]]
[[Category: Glycosidase]]
[[Category: Glycosidase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:30:40 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:31:04 2008''

Revision as of 00:31, 1 July 2008

File:1fmp.png

Template:STRUCTURE 1fmp

X-RAY ANALYSIS OF SUBSTRATE ANALOGS IN THE RICIN A CHAIN ACTIVE SITE

Template:ABSTRACT PUBMED 1433290

About this Structure

1FMP is a Single protein structure of sequence from Ricinus communis. Full crystallographic information is available from OCA.

Reference

X-ray analysis of substrate analogs in the ricin A-chain active site., Monzingo AF, Robertus JD, J Mol Biol. 1992 Oct 20;227(4):1136-45. PMID:1433290

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