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| [[Image:1fmu.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1fmu| PDB=1fmu | SCENE= }} | | {{STRUCTURE_1fmu| PDB=1fmu | SCENE= }} |
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| '''STRUCTURE OF NATIVE PROTEINASE A IN P3221 SPACE GROUP.'''
| | ===STRUCTURE OF NATIVE PROTEINASE A IN P3221 SPACE GROUP.=== |
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| ==Overview==
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| The structures of the native Saccharomyces cerevisiae proteinase A have been solved by molecular replacement in the monoclinic and trigonal crystal forms and refined at 2.6-2.7A resolution. These structures agree overall with those of other uninhibited aspartic proteinases. However, an unusual orientation for the side chain of Tyr75, a conserved residue on the flexible "flap" that covers the active site and is important for the activity of these enzymes, was found in the trigonal crystals. A similar conformation of Tyr75 occupying the S1 substrate-binding pocket was previously reported only for chymosin (where it was interpreted as representing a "self-inhibited" state of the enzyme), but for no other aspartic proteinases. Since this orientation of Tyr75 has now been seen in the structures of two members of the family of aspartic proteinases, it might indicate that the placement of that residue in the S1 substrate-binding pocket might have some functional significance, analogous to what was seen for self-inhibited structures of serine proteinases. | | The line below this paragraph, {{ABSTRACT_PUBMED_12127998}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12127998 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12127998}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Wlodawer, A.]] | | [[Category: Wlodawer, A.]] |
| [[Category: Proteinase some]] | | [[Category: Proteinase some]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:30:56 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:32:03 2008'' |
