1fo2: Difference between revisions

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[[Image:1fo2.jpg|left|200px]]
{{Seed}}
[[Image:1fo2.png|left|200px]]


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{{STRUCTURE_1fo2|  PDB=1fo2  |  SCENE=  }}  
{{STRUCTURE_1fo2|  PDB=1fo2  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF HUMAN CLASS I ALPHA1,2-MANNOSIDASE IN COMPLEX WITH 1-DEOXYMANNOJIRIMYCIN'''
===CRYSTAL STRUCTURE OF HUMAN CLASS I ALPHA1,2-MANNOSIDASE IN COMPLEX WITH 1-DEOXYMANNOJIRIMYCIN===




==Overview==
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Endoplasmic reticulum (ER) class I alpha1,2-mannosidase (also known as ER alpha-mannosidase I) is a critical enzyme in the maturation of N-linked oligosaccharides and ER-associated degradation. Trimming of a single mannose residue acts as a signal to target misfolded glycoproteins for degradation by the proteasome. Crystal structures of the catalytic domain of human ER class I alpha1,2-mannosidase have been determined both in the presence and absence of the potent inhibitors kifunensine and 1-deoxymannojirimycin. Both inhibitors bind to the protein at the bottom of the active-site cavity, with the essential calcium ion coordinating the O-2' and O-3' hydroxyls and stabilizing the six-membered rings of both inhibitors in a (1)C(4) conformation. This is the first direct evidence of the role of the calcium ion. The lack of major conformational changes upon inhibitor binding and structural comparisons with the yeast alpha1, 2-mannosidase enzyme-product complex suggest that this class of inverting enzymes has a novel catalytic mechanism. The structures also provide insight into the specificity of this class of enzymes and provide a blueprint for the future design of novel inhibitors that prevent degradation of misfolded proteins in genetic diseases.
The line below this paragraph, {{ABSTRACT_PUBMED_10995765}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_10995765}}


==About this Structure==
==About this Structure==
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[[Category: Vallee, F.]]
[[Category: Vallee, F.]]
[[Category: Alpha-alpha7 barrel]]
[[Category: Alpha-alpha7 barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:33:37 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:39:28 2008''

Revision as of 00:39, 1 July 2008

File:1fo2.png

Template:STRUCTURE 1fo2

CRYSTAL STRUCTURE OF HUMAN CLASS I ALPHA1,2-MANNOSIDASE IN COMPLEX WITH 1-DEOXYMANNOJIRIMYCIN

Template:ABSTRACT PUBMED 10995765

About this Structure

1FO2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis for catalysis and inhibition of N-glycan processing class I alpha 1,2-mannosidases., Vallee F, Karaveg K, Herscovics A, Moremen KW, Howell PL, J Biol Chem. 2000 Dec 29;275(52):41287-98. PMID:10995765

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