1fov: Difference between revisions

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[[Image:1fov.gif|left|200px]]
{{Seed}}
[[Image:1fov.png|left|200px]]


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{{STRUCTURE_1fov|  PDB=1fov  |  SCENE=  }}  
{{STRUCTURE_1fov|  PDB=1fov  |  SCENE=  }}  


'''GLUTAREDOXIN 3 FROM ESCHERICHIA COLI IN THE FULLY OXIDIZED FORM'''
===GLUTAREDOXIN 3 FROM ESCHERICHIA COLI IN THE FULLY OXIDIZED FORM===




==Overview==
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A high precision NMR structure of oxidized glutaredoxin 3 [C65Y] from Escherichia coli has been determined. The conformation of the active site including the disulphide bridge is highly similar to those in glutaredoxins from pig liver and T4 phage. A comparison with the previously determined structure of glutaredoxin 3 [C14S, C65Y] in a complex with glutathione reveals conformational changes between the free and substrate-bound form which includes the sidechain of the conserved, active site tyrosine residue. In the oxidized form this tyrosine is solvent exposed, while it adopts a less exposed conformation, stabilized by hydrogen bonds, in the mixed disulfide with glutathione. The structures further suggest that the formation of a covalent linkage between glutathione and glutaredoxin 3 is necessary in order to induce these structural changes upon binding of the glutathione peptide. This could explain the observed low affinity of glutaredoxins for S-blocked glutathione analogues, in spite of the fact that glutaredoxins are highly specific reductants of glutathione mixed disulfides.
The line below this paragraph, {{ABSTRACT_PUBMED_11031118}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 11031118 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11031118}}


==About this Structure==
==About this Structure==
1FOV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOV OCA].  
1FOV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FOV OCA].  


==Reference==
==Reference==
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[[Category: Active site disulfide]]
[[Category: Active site disulfide]]
[[Category: Cis pro 53]]
[[Category: Cis pro 53]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:35:18 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:42:21 2008''

Revision as of 00:42, 1 July 2008

File:1fov.png

Template:STRUCTURE 1fov

GLUTAREDOXIN 3 FROM ESCHERICHIA COLI IN THE FULLY OXIDIZED FORM

Template:ABSTRACT PUBMED 11031118

About this Structure

1FOV is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

NMR structure of oxidized glutaredoxin 3 from Escherichia coli., Nordstrand K, Sandstrom A, Aslund F, Holmgren A, Otting G, Berndt KD, J Mol Biol. 2000 Oct 27;303(3):423-32. PMID:11031118

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