1fre: Difference between revisions

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[[Image:1fre.gif|left|200px]]
{{Seed}}
[[Image:1fre.png|left|200px]]


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{{STRUCTURE_1fre|  PDB=1fre  |  SCENE=  }}  
{{STRUCTURE_1fre|  PDB=1fre  |  SCENE=  }}  


'''XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE'''
===XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE===




==Overview==
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Xenopus nuclear factor XNF7, a maternally expressed protein, functions in patterning of the embryo. XNF7 contains a number of defined protein domains implicated in the regulation of some developmental processes. Among these is a tripartite motif comprising a zinc-binding RING finger and B-box domain next to a predicted alpha-helical coiled-coil domain. Interestingly, this motif is found in a variety of protein including several proto-oncoproteins. Here we describe the solution structure of the XNF7 B-box zinc-binding domain determined at physiological pH by 1H NMR methods. The B-box structure represents the first three-dimensional structure of this new motif and comprises a monomer have two beta-strands, two helical turns and three extended loop regions packed in a novel topology. The r.m.s. deviation for the best 18 structures is 1.15 A for backbone atoms and 1.94 A for all atoms. Structure calculations and biochemical data shows one zinc atom ligated in a Cys2-His2 tetrahedral arrangement. We have used mutant peptides to determine the metal ligation scheme which surprisingly shows that not all of the seven conserved cysteines/histidines in the B-box motif are involved in metal ligation. The B-box structure is not similar in tertiary fold to any other known zinc-binding motif.
The line below this paragraph, {{ABSTRACT_PUBMED_8846787}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_8846787}}


==About this Structure==
==About this Structure==
1FRE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRE OCA].  
1FRE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FRE OCA].  


==Reference==
==Reference==
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[[Category: Xnf7]]
[[Category: Xnf7]]
[[Category: Zinc-binding protein]]
[[Category: Zinc-binding protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:40:53 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:49:46 2008''

Revision as of 00:49, 1 July 2008

File:1fre.png

Template:STRUCTURE 1fre

XNF7 BBOX, DEVELOPMENTAL PROTEIN, PH 7.5, 30 C, WITH ZINC, NMR, 1 STRUCTURE

Template:ABSTRACT PUBMED 8846787

About this Structure

1FRE is a Single protein structure of sequence from Xenopus laevis. Full experimental information is available from OCA.

Reference

Novel topology of a zinc-binding domain from a protein involved in regulating early Xenopus development., Borden KL, Lally JM, Martin SR, O'Reilly NJ, Etkin LD, Freemont PS, EMBO J. 1995 Dec 1;14(23):5947-56. PMID:8846787

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