1fsi: Difference between revisions

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[[Image:1fsi.gif|left|200px]]
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[[Image:1fsi.png|left|200px]]


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{{STRUCTURE_1fsi|  PDB=1fsi  |  SCENE=  }}  
{{STRUCTURE_1fsi|  PDB=1fsi  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE OF APPR>P FROM ARABIDOPSIS THALIANA'''
===CRYSTAL STRUCTURE OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE OF APPR>P FROM ARABIDOPSIS THALIANA===




==Overview==
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The crystal structure of the cyclic phosphodiesterase (CPDase) from Arabidopsis thaliana, an enzyme involved in the tRNA splicing pathway, was determined at 2.5 A resolution. CPDase hydrolyzes ADP-ribose 1",2"-cyclic phosphate (Appr&gt;p), a product of the tRNA splicing reaction, to the monoester ADP-ribose 1"-phosphate (Appr-1"p). The 181 amino acid protein shows a novel, bilobal arrangement of two alphabeta modules. Each lobe consists of two alpha-helices on the outer side of the molecule, framing a three- or four-stranded antiparallel beta-sheet in the core of the protein. The active site is formed at the interface of the two beta-sheets in a water-filled cavity involving residues from two H-X-T/S-X motifs. This previously noticed motif participates in coordination of a sulfate ion. A solvent-exposed surface loop (residues 100-115) is very likely to play a flap-like role, opening and closing the active site. Based on the crystal structure and on recent mutagenesis studies of a homologous CPDase from Saccharomyces cerevisiae, we propose an enzymatic mechanism that employs the nucleophilic attack of a water molecule activated by one of the active site histidines.
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{{ABSTRACT_PUBMED_11080166}}


==About this Structure==
==About this Structure==
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[[Category: Adp-ribose 1'',2''-cyclic phosphate]]
[[Category: Adp-ribose 1'',2''-cyclic phosphate]]
[[Category: Appr>p]]
[[Category: Appr>p]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:42:51 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:52:49 2008''

Revision as of 00:52, 1 July 2008

File:1fsi.png

Template:STRUCTURE 1fsi

CRYSTAL STRUCTURE OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE OF APPR>P FROM ARABIDOPSIS THALIANA

Template:ABSTRACT PUBMED 11080166

About this Structure

1FSI is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of activity of the cyclic phosphodiesterase of Appr>p, a product of the tRNA splicing reaction., Hofmann A, Zdanov A, Genschik P, Ruvinov S, Filipowicz W, Wlodawer A, EMBO J. 2000 Nov 15;19(22):6207-17. PMID:11080166 [[Category: Appr>p]]

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