1g1j: Difference between revisions

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[[Image:1g1j.gif|left|200px]]
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[[Image:1g1j.png|left|200px]]


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{{STRUCTURE_1g1j|  PDB=1g1j  |  SCENE=  }}  
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'''CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4'''
===CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4===




==Overview==
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During the maturation of rotaviral particles, non-structural protein 4 (NSP4) plays a critical role in the translocation of the immature capsid into the lumen of the endoplasmic reticulum. Full-length NSP4 and a 22 amino acid peptide (NSP4(114-135)) derived from this protein have been shown to induce diarrhea in young mice in an age-dependent manner, and may therefore be the agent responsible for rotavirally-induced symptoms. We have determined the crystal structure of the oligomerization domain of NSP4 which spans residues 95 to 137 (NSP4(95-137)). NSP4(95-137) self-associates into a parallel, tetrameric coiled-coil, with the hydrophobic core interrupted by three polar layers occupying a and d-heptad positions. Side-chains from two consecutive polar layers, consisting of four Gln123 and two of the four Glu120 residues, coordinate a divalent cation. Two independent structures built from MAD-phased data indicated the presence of a strontium and calcium ion bound at this site, respectively. This metal-binding site appears to play an important role in stabilizing the homo-tetramer, which has implications for the engagement of NSP4 as an enterotoxin.
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{{ABSTRACT_PUBMED_11124032}}


==About this Structure==
==About this Structure==
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[[Category: Parallel coiled-coil]]
[[Category: Parallel coiled-coil]]
[[Category: Strontium]]
[[Category: Strontium]]
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Revision as of 01:16, 1 July 2008

File:1g1j.png

Template:STRUCTURE 1g1j

CRYSTAL STRUCTURE OF THE OLIGOMERIZATION DOMAIN FROM ROTAVIRUS NSP4

Template:ABSTRACT PUBMED 11124032

About this Structure

1G1J is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Crystal structure of the oligomerization domain of NSP4 from rotavirus reveals a core metal-binding site., Bowman GD, Nodelman IM, Levy O, Lin SL, Tian P, Zamb TJ, Udem SA, Venkataraghavan B, Schutt CE, J Mol Biol. 2000 Dec 15;304(5):861-71. PMID:11124032

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