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| {{STRUCTURE_1g63| PDB=1g63 | SCENE= }} | | {{STRUCTURE_1g63| PDB=1g63 | SCENE= }} |
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| '''PEPTIDYL-CYSTEINE DECARBOXYLASE EPID'''
| | ===PEPTIDYL-CYSTEINE DECARBOXYLASE EPID=== |
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| ==Overview==
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| Epidermin from Staphylococcus epidermidis Tu3298 is an antimicrobial peptide of the lantibiotic family that contains, amongst other unusual amino acids, S:-[(Z:)- 2-aminovinyl]-D-cysteine. This residue is introduced by post-translational modification of the ribosomally synthesized precursor EpiA. Modification starts with the oxidative decarboxylation of its C-terminal cysteine by the flavoprotein EpiD generating a reactive (Z:)-enethiol intermediate. We have determined the crystal structures of EpiD and EpiD H67N in complex with the substrate pentapeptide DSYTC at 2.5 A resolution. Rossmann-type monomers build up a dodecamer of 23 point symmetry with trimers disposed at the vertices of a tetrahedron. Oligomer formation is essential for binding of flavin mononucleotide and substrate, which is buried by an otherwise disordered substrate recognition clamp. A pocket for the tyrosine residue of the substrate peptide is formed by an induced fit mechanism. The substrate contacts flavin mononucleotide only via Cys-Sgamma, suggesting its oxidation as the initial step. A thioaldehyde intermediate could undergo spontaneous decarboxylation. The unusual substrate recognition mode and the type of chemical reaction performed provide insight into a novel family of flavoproteins.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11101502}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11101502 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11101502}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Beta protein]] | | [[Category: Beta protein]] |
| [[Category: Rossmann like fold]] | | [[Category: Rossmann like fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:11:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:32:10 2008'' |
