1gcy: Difference between revisions

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[[Image:1gcy.gif|left|200px]]
{{Seed}}
[[Image:1gcy.png|left|200px]]


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{{STRUCTURE_1gcy|  PDB=1gcy  |  SCENE=  }}  
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'''HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE'''
===HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE===




==Overview==
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The intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri (G4-1), which has a raw starch binding domain, has been crystallized. The structure was identified (PDB entry 1GCY) by the molecular replacement method using the structure of its catalytic domain (G4-2). The result showed that the raw starch binding domain is in a disordered state, the corresponding electron densities being almost invisible. Superposition of these two enzyme forms showed evidence for the possible location of the raw starch binding domain (SBD). This crystal is a novel case, in that it forms a regular lattice incorporating flexibly bound SBD in the channel of crystal packing of the catalytic domains.
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{{ABSTRACT_PUBMED_11272837}}


==About this Structure==
==About this Structure==
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[[Category: Beta sheet]]
[[Category: Beta sheet]]
[[Category: Beta-alpha-barrel]]
[[Category: Beta-alpha-barrel]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:25:35 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:06:35 2008''

Revision as of 02:06, 1 July 2008

File:1gcy.png

Template:STRUCTURE 1gcy

HIGH RESOLUTION CRYSTAL STRUCTURE OF MALTOTETRAOSE-FORMING EXO-AMYLASE

Template:ABSTRACT PUBMED 11272837

About this Structure

1GCY is a Single protein structure of sequence from Pseudomonas stutzeri. Full crystallographic information is available from OCA.

Reference

Crystallization and structural analysis of intact maltotetraose-forming exo-amylase from Pseudomonas stutzeri., Mezaki Y, Katsuya Y, Kubota M, Matsuura Y, Biosci Biotechnol Biochem. 2001 Jan;65(1):222-5. PMID:11272837

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