1gd4: Difference between revisions

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[[Image:1gd4.gif|left|200px]]
{{Seed}}
[[Image:1gd4.png|left|200px]]


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{{STRUCTURE_1gd4|  PDB=1gd4  |  SCENE=  }}  
{{STRUCTURE_1gd4|  PDB=1gd4  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF P25S CYSTATIN A'''
===SOLUTION STRUCTURE OF P25S CYSTATIN A===




==Overview==
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The effect of substituting Pro25, located in the alpha-helical region of the cystatin A structure, with Ser has been studied. The structures of wild type and P25S cystatin A were determined by multidimensional NMR spectroscopy under comparable conditions. These two structures were virtually identical, and the alpha-helix between Glu15-Lys30 exists with uninterrupted continuity, with a slight bend at residue 25. In order to characterize the possible substitution effects of Pro25 with Ser on the alpha-helix, the chemical shifts of the amide nitrogens and protons, the generalized order parameters obtained by the analyses of the 15N-1H relaxation data, the amide proton exchange rates, and the NOE networks among the alpha-helical and surrounding residues were carefully compared. None of these parameters indicated any significant static or dynamic structural differences between the alpha-helical regions of the wild-type and P25S cystatin A proteins. We therefore conclude that our previous structure of the wild-type cystatin A, in which the alpha-helix exhibited a sharp kink at Pro25, must be revised. The asymmetric distribution of hydrophobic interactions between the side-chain residues of the alpha-helix and the rolled beta-sheet surface, as revealed by NOEs, may be responsible for the slight bend of the alpha-helix in both variants and for the destabilized hydrogen bonding of the alpha-helical residues that follow Pro25/Ser25, as evidenced by increased amide exchange rates.
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{{ABSTRACT_PUBMED_12836678}}


==About this Structure==
==About this Structure==
1GD4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GD4 OCA].  
1GD4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GD4 OCA].  


==Reference==
==Reference==
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[[Category: Cystatin some]]
[[Category: Cystatin some]]
[[Category: Thiol protease inhibitor]]
[[Category: Thiol protease inhibitor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:26:01 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:07:16 2008''

Revision as of 02:07, 1 July 2008

File:1gd4.png

Template:STRUCTURE 1gd4

SOLUTION STRUCTURE OF P25S CYSTATIN A

Template:ABSTRACT PUBMED 12836678

About this Structure

1GD4 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Structural comparison between wild-type and P25S human cystatin A by NMR spectroscopy. Does this mutation affect the alpha-helix conformation?, Shimba N, Kariya E, Tate S, Kaji H, Kainosho M, J Struct Funct Genomics. 2000;1(1):26-42. PMID:12836678

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