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| [[Image:1gd3.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1gd3| PDB=1gd3 | SCENE= }} | | {{STRUCTURE_1gd3| PDB=1gd3 | SCENE= }} |
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| '''refined solution structure of human cystatin A'''
| | ===refined solution structure of human cystatin A=== |
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| ==Overview==
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| The effect of substituting Pro25, located in the alpha-helical region of the cystatin A structure, with Ser has been studied. The structures of wild type and P25S cystatin A were determined by multidimensional NMR spectroscopy under comparable conditions. These two structures were virtually identical, and the alpha-helix between Glu15-Lys30 exists with uninterrupted continuity, with a slight bend at residue 25. In order to characterize the possible substitution effects of Pro25 with Ser on the alpha-helix, the chemical shifts of the amide nitrogens and protons, the generalized order parameters obtained by the analyses of the 15N-1H relaxation data, the amide proton exchange rates, and the NOE networks among the alpha-helical and surrounding residues were carefully compared. None of these parameters indicated any significant static or dynamic structural differences between the alpha-helical regions of the wild-type and P25S cystatin A proteins. We therefore conclude that our previous structure of the wild-type cystatin A, in which the alpha-helix exhibited a sharp kink at Pro25, must be revised. The asymmetric distribution of hydrophobic interactions between the side-chain residues of the alpha-helix and the rolled beta-sheet surface, as revealed by NOEs, may be responsible for the slight bend of the alpha-helix in both variants and for the destabilized hydrogen bonding of the alpha-helical residues that follow Pro25/Ser25, as evidenced by increased amide exchange rates. | | The line below this paragraph, {{ABSTRACT_PUBMED_12836678}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12836678 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12836678}} |
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| ==About this Structure== | | ==About this Structure== |
| 1GD3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GD3 OCA]. | | 1GD3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GD3 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Cystatin some]] | | [[Category: Cystatin some]] |
| [[Category: Thiol protease inhibitor]] | | [[Category: Thiol protease inhibitor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:26:01 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:07:15 2008'' |
