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| {{STRUCTURE_1gl7| PDB=1gl7 | SCENE= }} | | {{STRUCTURE_1gl7| PDB=1gl7 | SCENE= }} |
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| '''PLASMID COUPLING PROTEIN TRWB IN COMPLEX WITH THE NON-HYDROLISABLE ATP-ANALOGUE ADPNP.'''
| | ===PLASMID COUPLING PROTEIN TRWB IN COMPLEX WITH THE NON-HYDROLISABLE ATP-ANALOGUE ADPNP.=== |
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| ==Overview==
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| The transfer of DNA across membranes and between cells is a central biological process; however, its molecular mechanism remains unknown. In prokaryotes, trans-membrane passage by bacterial conjugation, is the main route for horizontal gene transfer. It is the means for rapid acquisition of new genetic information, including antibiotic resistance by pathogens. Trans-kingdom gene transfer from bacteria to plants or fungi and even bacterial sporulation are special cases of conjugation. An integral membrane DNA-binding protein, called TrwB in the Escherichia coli R388 conjugative system, is essential for the conjugation process. This large multimeric protein is responsible for recruiting the relaxosome DNA-protein complex, and participates in the transfer of a single DNA strand during cell mating. Here we report the three-dimensional structure of a soluble variant of TrwB. The molecule consists of two domains: a nucleotide-binding domain of alpha/beta topology, reminiscent of RecA and DNA ring helicases, and an all-alpha domain. Six equivalent protein monomers associate to form an almost spherical quaternary structure that is strikingly similar to F1-ATPase. A central channel, 20 A in width, traverses the hexamer. | | The line below this paragraph, {{ABSTRACT_PUBMED_11214325}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11214325 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11214325}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Moncalian, G.]] | | [[Category: Moncalian, G.]] |
| [[Category: Type iv secretion system conjugative coupling protein from plasmid]] | | [[Category: Type iv secretion system conjugative coupling protein from plasmid]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:43:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:28:19 2008'' |
