1gql: Difference between revisions

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~~'''~~STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE COMPLEXED WITH GLUCURONIC ACID AND XYLOTRIOSE~~'''~~

===STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE COMPLEXED WITH GLUCURONIC ACID AND XYLOTRIOSE===

~~==Overview==~~

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~~Alpha~~-~~glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha~~-~~1,2 linked 4-O-methyl glucuronic acid from xylans.~~ The ~~structure of the alpha-glucuronidase~~, ~~GlcA67A, from Pseudomonas cellulosa reveals three domains~~, the ~~central of which is a~~ (~~beta~~/~~alpha)(8~~) ~~barrel housing the catalytic apparatus. Complexes of the enzyme with the individual reaction products~~, ~~either xylobiose or glucuronic acid, and~~ the ternary complex of both glucuronic acid and xylotriose reveal a "blind" pocket which selects for short decorated xylooligosaccharides substituted with the uronic acid at their nonreducing end, consistent with kinetic data. The catalytic center reveals a constellation of carboxylates; Glu292 is poised to provide protonic assistance to leaving group departure with Glu393 and Asp365 both appropriately positioned to provide base-~~catalyzed assistance for inverting nucleophilic attack by water.~~

The line below this paragraph, {{ABSTRACT_PUBMED_11937059}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 11937059 is the PubMed ID number.

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==About this Structure==

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[[Category: Structure]]

[[Category: Xylotriose]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Fri May~~ ~~2 17~~:53:45 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:54:22 2008''

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-[[Image:1gql.gif|left|200px]]
+{{Seed}}
+[[Image:1gql.png|left|200px]]
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 {{STRUCTURE_1gql|  PDB=1gql  |  SCENE=  }}
-'''STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE COMPLEXED WITH GLUCURONIC ACID AND XYLOTRIOSE'''
+===STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE COMPLEXED WITH GLUCURONIC ACID AND XYLOTRIOSE===
-==Overview==
+<!--
-Alpha-glucuronidases, components of an ensemble of enzymes central to the recycling of photosynthetic biomass, remove the alpha-1,2 linked 4-O-methyl glucuronic acid from xylans. The structure of the alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three domains, the central of which is a (beta/alpha)(8) barrel housing the catalytic apparatus. Complexes of the enzyme with the individual reaction products, either xylobiose or glucuronic acid, and the ternary complex of both glucuronic acid and xylotriose reveal a "blind" pocket which selects for short decorated xylooligosaccharides substituted with the uronic acid at their nonreducing end, consistent with kinetic data. The catalytic center reveals a constellation of carboxylates; Glu292 is poised to provide protonic assistance to leaving group departure with Glu393 and Asp365 both appropriately positioned to provide base-catalyzed assistance for inverting nucleophilic attack by water.
+The line below this paragraph, {{ABSTRACT_PUBMED_11937059}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 11937059 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_11937059}}
 ==About this Structure==
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 [[Category: Structure]]
 [[Category: Xylotriose]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 17:53:45 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul  1 05:54:22 2008''