1h2o: Difference between revisions

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[[Image:1h2o.jpg|left|200px]]
{{Seed}}
[[Image:1h2o.png|left|200px]]


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{{STRUCTURE_1h2o|  PDB=1h2o  |  SCENE=  }}  
{{STRUCTURE_1h2o|  PDB=1h2o  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W'''
===SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W===




==Overview==
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Birch pollinosis is often accompanied by adverse reactions to food due to pollen-allergen specific IgE cross-reacting with homologous food allergens. The tertiary structure of Pru av 1, the major cherry (Prunus avium) allergen, for example, is nearly identical with Bet v 1, the major birch (Betula verrucosa) pollen allergen. In order to define cross-reactive IgE epitopes, we generated and analysed mutants of Pru av 1 and Api g 1.0101, the major celery (Apium graveolens) allergen, by immunoblotting, EAST (enzyme allergosorbent test), CD and NMR spectroscopy. The mutation of Glu45 to Trp45 in the P-loop region, a known IgE epitope of Bet v 1, significantly reduced IgE binding to Pru av 1 in a subgroup of cherry-allergic patients. The backbone conformation of Pru av 1 wild-type is conserved in the three-dimensional structure of Pru av 1 Trp45, demonstrating that the side chain of Glu45 is involved in a cross-reactive IgE epitope. Accordingly, for a subgroup of celery-allergic patients, IgE binding to the homologous celery allergen Api g 1.0101 was enhanced by the mutation of Lys44 to Glu. The almost complete loss of IgE reactivity to the Pru av 1 Pro112 mutant is due to disruption of its tertiary structure. Neither the mutation Ala112 nor deletion of the C-terminal residues 155-159 influenced IgE binding to Pru av 1. In conclusion, the structure of the P-loop partially explains the cross-reactivity pattern, and modulation of IgE-binding by site-directed mutagenesis is a promising approach to develop hypo-allergenic variants for patient-tailored specific immunotherapy.
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{{ABSTRACT_PUBMED_12943529}}


==About this Structure==
==About this Structure==
1H2O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Prunus_avium Prunus avium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2O OCA].  
1H2O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Prunus_avium Prunus avium]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2O OCA].  


==Reference==
==Reference==
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[[Category: Plant defense]]
[[Category: Plant defense]]
[[Category: Structure]]
[[Category: Structure]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:20:58 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:31:25 2008''

Revision as of 03:31, 1 July 2008

File:1h2o.png

Template:STRUCTURE 1h2o

SOLUTION STRUCTURE OF THE MAJOR CHERRY ALLERGEN PRU AV 1 MUTANT E45W

Template:ABSTRACT PUBMED 12943529

About this Structure

1H2O is a Single protein structure of sequence from Prunus avium. Full experimental information is available from OCA.

Reference

Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens): correlating IgE reactivity with three-dimensional structure., Neudecker P, Lehmann K, Nerkamp J, Haase T, Wangorsch A, Fotisch K, Hoffmann S, Rosch P, Vieths S, Scheurer S, Biochem J. 2003 Nov 15;376(Pt 1):97-107. PMID:12943529

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