1hcb: Difference between revisions

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[[Image:1hcb.gif|left|200px]]
{{Seed}}
[[Image:1hcb.png|left|200px]]


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{{STRUCTURE_1hcb|  PDB=1hcb  |  SCENE=  }}  
{{STRUCTURE_1hcb|  PDB=1hcb  |  SCENE=  }}  


'''ENZYME-SUBSTRATE INTERACTIONS: STRUCTURE OF HUMAN CARBONIC ANHYDRASE I COMPLEXED WITH BICARBONATE'''
===ENZYME-SUBSTRATE INTERACTIONS: STRUCTURE OF HUMAN CARBONIC ANHYDRASE I COMPLEXED WITH BICARBONATE===




==Overview==
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The structure of HCAI-HCO3- complex has been refined with 10-1.6A X-ray diffraction data to an R-value of 17.7%. The structure reveals monodentate binding of the HCO3- anion at an apical tetrahedral position to the zinc ion. The binding mode and interactions of HCO3- in HCAI differ from that in HCAII. The activity linked H2O/OH- group in the free HCAI is replaced by the hydroxyl group of the bicarbonate anion. This result rules out the rearrangement of the bound HCO3- advocated earlier to explain the microscopic reversibility of the catalysed reaction. From the geometry of the H-bonds between Glu106-Thr199 pair and Glu117-His119 couple, the glutamic acids are expected to be ionized and accept H-bonds from their partners. The product-inhibiton by HCO3- anion is explained on the basis of proton localization on His119 in the Glu117-His119 couple. These results are consistent with the hypothesis that Glu117-His119 tunes the ionicity of the Zn2+ and the binding strength of HCO3- anion. A pi hydrogen bond is observed between a water and phenyl ring of the Tyr114 residue.
The line below this paragraph, {{ABSTRACT_PUBMED_8057362}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 8057362 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8057362}}


==About this Structure==
==About this Structure==
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[[Category: Kannan, K K.]]
[[Category: Kannan, K K.]]
[[Category: Kumar, V.]]
[[Category: Kumar, V.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:41:28 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 07:58:41 2008''

Revision as of 04:58, 1 July 2008

File:1hcb.png

Template:STRUCTURE 1hcb

ENZYME-SUBSTRATE INTERACTIONS: STRUCTURE OF HUMAN CARBONIC ANHYDRASE I COMPLEXED WITH BICARBONATE

Template:ABSTRACT PUBMED 8057362

About this Structure

1HCB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Enzyme-substrate interactions. Structure of human carbonic anhydrase I complexed with bicarbonate., Kumar V, Kannan KK, J Mol Biol. 1994 Aug 12;241(2):226-32. PMID:8057362

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