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| [[Image:1hi1.gif|left|200px]] | | {{Seed}} |
| | [[Image:1hi1.png|left|200px]] |
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| {{STRUCTURE_1hi1| PDB=1hi1 | SCENE= }} | | {{STRUCTURE_1hi1| PDB=1hi1 | SCENE= }} |
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| '''RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS BOUND NTP'''
| | ===RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS BOUND NTP=== |
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| ==Overview==
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| In most RNA viruses, genome replication and transcription are catalysed by a viral RNA-dependent RNA polymerase. Double-stranded RNA viruses perform these operations in a capsid (the polymerase complex), using an enzyme that can read both single- and double-stranded RNA. Structures have been solved for such viral capsids, but they do not resolve the polymerase subunits in any detail. Here we show that the 2 A resolution X-ray structure of the active polymerase subunit from the double-stranded RNA bacteriophage straight phi6 is highly similar to that of the polymerase of hepatitis C virus, providing an evolutionary link between double-stranded RNA viruses and flaviviruses. By crystal soaking and co-crystallization, we determined a number of other structures, including complexes with oligonucleotide and/or nucleoside triphosphates (NTPs), that suggest a mechanism by which the incoming double-stranded RNA is opened up to feed the template through to the active site, while the substrates enter by another route. The template strand initially overshoots, locking into a specificity pocket, and then, in the presence of cognate NTPs, reverses to form the initiation complex; this process engages two NTPs, one of which acts with the carboxy-terminal domain of the protein to prime the reaction. Our results provide a working model for the initiation of replication and transcription.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11242087}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11242087 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11242087}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Rna polymerase]] | | [[Category: Rna polymerase]] |
| [[Category: Viral polymerase]] | | [[Category: Viral polymerase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:51:57 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:12:42 2008'' |
