1ho0: Difference between revisions

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[[Image:1ho0.jpg|left|200px]]
{{Seed}}
[[Image:1ho0.png|left|200px]]


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{{STRUCTURE_1ho0|  PDB=1ho0  |  SCENE=  }}  
{{STRUCTURE_1ho0|  PDB=1ho0  |  SCENE=  }}  


'''NEW B-CHAIN MUTANT OF BOVINE INSULIN'''
===NEW B-CHAIN MUTANT OF BOVINE INSULIN===




==Overview==
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The solution structure of a new B-chain mutant of bovine insulin, in which the cysteines B7 and B19 are replaced by two serines, has been determined by circular dichroism, 2D-NMR and molecular modeling. This structure is compared with that of the oxidized B-chain of bovine insulin [Hawkins et al. (1995) Int. J. Peptide Protein Res.46, 424-433]. Circular dichroism spectroscopy showed in particular that a higher percentage of helical secondary structure for the B-chain mutant is estimated in trifluoroethanol solution in comparison with the oxidized B-chain. 2D-NMR experiments confirmed, among multiple conformations, that the B-chain mutant presents defined secondary structures such as a alpha-helix between residues B9 and B19, and a beta-turn between amino acids B20 and B23 in aqueous trifluoroethanol. The 3D structures, which are consistent with NMR data and were obtained using a simulated annealing protocol, showed that the tertiary structure of the B-chain mutant is better resolved and is more in agreement with the insulin crystal structure than the oxidized B-chain structure described by Hawkins et al. An explanation could be the presence of two sulfonate groups in the oxidized insulin B-chain. Either by their charges and/or their size, such chemical groups could play a destructuring effect and thus could favor peptide flexibility and conformational averaging. Thus, this study provides new insights on the folding of isolated B-chains.
The line below this paragraph, {{ABSTRACT_PUBMED_12081626}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12081626 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12081626}}


==About this Structure==
==About this Structure==
1HO0 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HO0 OCA].  
1HO0 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HO0 OCA].  


==Reference==
==Reference==
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[[Category: Prigent, Y.]]
[[Category: Prigent, Y.]]
[[Category: Richard, T.]]
[[Category: Richard, T.]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:03:20 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:27:11 2008''

Revision as of 05:27, 1 July 2008

File:1ho0.png

Template:STRUCTURE 1ho0

NEW B-CHAIN MUTANT OF BOVINE INSULIN

Template:ABSTRACT PUBMED 12081626

About this Structure

1HO0 is a Single protein structure. Full experimental information is available from OCA.

Reference

A new B-chain mutant of insulin: comparison with the insulin crystal structure and role of sulfonate groups in the B-chain structure., Dupradeau FY, Richard T, Le Flem G, Oulyadi H, Prigent Y, Monti JP, J Pept Res. 2002 Jul;60(1):56-64. PMID:12081626

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