1hp2: Difference between revisions

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[[Image:1hp2.jpg|left|200px]]
{{Seed}}
[[Image:1hp2.png|left|200px]]


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{{STRUCTURE_1hp2|  PDB=1hp2  |  SCENE=  }}  
{{STRUCTURE_1hp2|  PDB=1hp2  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF A TOXIN FROM THE SCORPION TITYUS SERRULATUS (TSTX-K ALPHA) DETERMINED BY NMR.'''
===SOLUTION STRUCTURE OF A TOXIN FROM THE SCORPION TITYUS SERRULATUS (TSTX-K ALPHA) DETERMINED BY NMR.===




==Overview==
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A toxin from the scorpion Tityus serrulatus (TsTX-Kalpha) blocks native squid K(+) channels and their cloned counterpart, sqKv1A, at pH 8 ((native)K(d) approximately 20 nM; (sqKv1A)K(d) approximately 10 nM). In both cases, decreasing the pH below 7.0 significantly diminishes the TsTX-Kalpha effect (pK = 6.6). In the cloned squid channel, the pH dependence of the block is abolished by a single point mutation (H351G), and no change in toxin affinity was observed at higher pH values (pH &gt; or =8.0). To further investigate the TsTX-Kalpha-sqKv1A interaction, the three-dimensional structure of TsTX-Kalpha was determined in solution by NMR spectroscopy, and a model of the TsTX-Kalpha-sqKv1A complex was generated. As found for other alpha-K toxins such as charybdotoxin (CTX), site-directed mutagenesis at toxin residue K27 (K27A, K27R, and K27E) significantly reduced the toxin's affinity for sqKv1A channels. This is consistent with the TsTX-Kalpha-sqKv1A model reported here, which has K27 of the toxin inserted into the ion conduction pathway of the K(+) channel. This toxin-channel model also illustrates a possible mechanism for the pH-dependent block whereby lysine residues from TsTX-Kalpha (K6 and K23) are repelled by protonated H351 on sqKv1A at low pH.
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{{ABSTRACT_PUBMED_11352729}}


==About this Structure==
==About this Structure==
1HP2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tityus_serrulatus Tityus serrulatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HP2 OCA].  
1HP2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Tityus_serrulatus Tityus serrulatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HP2 OCA].  


==Reference==
==Reference==
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[[Category: K+ channel]]
[[Category: K+ channel]]
[[Category: Scorpion toxin]]
[[Category: Scorpion toxin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:04:59 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:29:22 2008''

Revision as of 05:29, 1 July 2008

File:1hp2.png

Template:STRUCTURE 1hp2

SOLUTION STRUCTURE OF A TOXIN FROM THE SCORPION TITYUS SERRULATUS (TSTX-K ALPHA) DETERMINED BY NMR.

Template:ABSTRACT PUBMED 11352729

About this Structure

1HP2 is a Single protein structure of sequence from Tityus serrulatus. Full experimental information is available from OCA.

Reference

Interaction of a toxin from the scorpion Tityus serrulatus with a cloned K+ channel from squid (sqKv1A)., Ellis KC, Tenenholz TC, Jerng H, Hayhurst M, Dudlak CS, Gilly WF, Blaustein MP, Weber DJ, Biochemistry. 2001 May 22;40(20):5942-53. PMID:11352729

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