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| [[Image:1hpu.gif|left|200px]] | | {{Seed}} |
| | [[Image:1hpu.png|left|200px]] |
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| {{STRUCTURE_1hpu| PDB=1hpu | SCENE= }} | | {{STRUCTURE_1hpu| PDB=1hpu | SCENE= }} |
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| '''5'-NUCLEOTIDASE (CLOSED FORM), COMPLEX WITH AMPCP'''
| | ===5'-NUCLEOTIDASE (CLOSED FORM), COMPLEX WITH AMPCP=== |
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| ==Overview==
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| 5'-Nucleotidase belongs to a large superfamily of distantly related dinuclear metallophosphatases including the Ser/Thr protein phosphatases and purple acid phosphatases. The protein undergoes a 96 degrees domain rotation between an open (inactive) and a closed (active) enzyme form. Complex structures of the closed form with the products adenosine and phosphate, and with the substrate analogue inhibitor alpha,beta-methylene ADP, have been determined at 2.1 A and 1.85 A resolution, respectively. In addition, a complex of the open form of 5'-nucleotidase with ATP was analyzed at a resolution of 1.7 A. These structures show that the adenosine group binds to a specific binding pocket of the C-terminal domain. The adenine ring is stacked between Phe429 and Phe498. The N-terminal domain provides the ligands to the dimetal cluster and the conserved His117, which together form the catalytic core structure. However, the three C-terminal arginine residues 375, 379 and 410, which are involved in substrate binding, may also play a role in transition-state stabilization. The beta-phosphate group of the inhibitor is terminally coordinated to the site 2 metal ion. The site 1 metal ion coordinates a water molecule which is in an ideal position for a nucleophilic attack on the phosphorus atom, assuming an in-line mechanism of phosphoryl transfer. Another water molecule bridges the two metal ions.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11491293}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11491293 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11491293}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Metalloenzyme]] | | [[Category: Metalloenzyme]] |
| [[Category: Metallophosphatase]] | | [[Category: Metallophosphatase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:06:07 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:30:48 2008'' |
