1hu6: Difference between revisions

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[[Image:1hu6.gif|left|200px]]
{{Seed}}
[[Image:1hu6.png|left|200px]]


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{{STRUCTURE_1hu6|  PDB=1hu6  |  SCENE=  }}  
{{STRUCTURE_1hu6|  PDB=1hu6  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF G10 NOVISPIRIN'''
===SOLUTION STRUCTURE OF G10 NOVISPIRIN===




==Overview==
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We studied three model antibacterial peptides that resembled the N-terminal 18 amino acids of SMAP-29, an alpha-helical, antimicrobial peptide of sheep. Although the parent compound, ovispirin-1 (KNLRR IIRKI IHIIK KYG), was potently antimicrobial, it was also highly cytotoxic to human epithelial cells and hemolytic for human erythrocytes. Single residue substitutions to ovispirin-1 yielded two substantially less cytotoxic peptides (novispirins), with intact antimicrobial properties. One of these, novispirin G-10, differed from ovispirin-1 only by containing glycine at position 10, instead of isoleucine. The other, novispirin T-7, contained threonine instead of isoleucine at position 7. We determined the three-dimensional solution structures of all three peptides by circular dichroism spectroscopy and two-dimensional nuclear magnetic resonance spectroscopy. Although all retained an amphipathic helical structure in 2,2,2-trifluoroethanol, they manifested subtle fine-structural changes that evidently impacted their activities greatly. These findings show that simple structural modifications can 'fine-tune' an antimicrobial peptide to minimize unwanted cytotoxicity while retaining its desired activity.
The line below this paragraph, {{ABSTRACT_PUBMED_11932493}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_11932493}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HU6 OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HU6 OCA].  


==Reference==
==Reference==
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[[Category: Peptide]]
[[Category: Peptide]]
[[Category: Solution structure]]
[[Category: Solution structure]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:13:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:40:46 2008''

Revision as of 05:40, 1 July 2008

File:1hu6.png

Template:STRUCTURE 1hu6

SOLUTION STRUCTURE OF G10 NOVISPIRIN

Template:ABSTRACT PUBMED 11932493

About this Structure

Full experimental information is available from OCA.

Reference

Impact of single-residue mutations on the structure and function of ovispirin/novispirin antimicrobial peptides., Sawai MV, Waring AJ, Kearney WR, McCray PB Jr, Forsyth WR, Lehrer RI, Tack BF, Protein Eng. 2002 Mar;15(3):225-32. PMID:11932493

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