1hwr: Difference between revisions

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[[Image:1hwr.gif|left|200px]]
{{Seed}}
[[Image:1hwr.png|left|200px]]


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{{STRUCTURE_1hwr|  PDB=1hwr  |  SCENE=  }}  
{{STRUCTURE_1hwr|  PDB=1hwr  |  SCENE=  }}  


'''MOLECULAR RECOGNITION OF CYCLIC UREA HIV PROTEASE INHIBITORS'''
===MOLECULAR RECOGNITION OF CYCLIC UREA HIV PROTEASE INHIBITORS===




==Overview==
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As long as the threat of human immunodeficiency virus (HIV) protease drug resistance still exists, there will be a need for more potent antiretroviral agents. We have therefore determined the crystal structures of HIV-1 protease in complex with six cyclic urea inhibitors: XK216, XK263, DMP323, DMP450, XV638, and SD146, in an attempt to identify 1) the key interactions responsible for their high potency and 2) new interactions that might improve their therapeutic benefit. The structures reveal that the preorganized, C2 symmetric scaffolds of the inhibitors are anchored in the active site of the protease by six hydrogen bonds and that their P1 and P2 substituents participate in extensive van der Waals interactions and hydrogen bonds. Because all of our inhibitors possess benzyl groups at P1 and P1', their relative binding affinities are modulated by the extent of their P2 interactions, e.g. XK216, the least potent inhibitor (Ki (inhibition constant) = 4.70 nM), possesses the smallest P2 and the lowest number of P2-S2 interactions; whereas SD146, the most potent inhibitor (Ki = 0.02 nM), contains a benzimidazolylbenzamide at P2 and participates in fourteen hydrogen bonds and approximately 200 van der Waals interactions. This analysis identifies the strongest interactions between the protease and the inhibitors, suggests ways to improve potency by building into the S2 subsite, and reveals how conformational changes and unique features of the viral protease increase the binding affinity of HIV protease inhibitors.
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{{ABSTRACT_PUBMED_9575185}}


==About this Structure==
==About this Structure==
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[[Category: Aspartyl protease]]
[[Category: Aspartyl protease]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:18:16 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:01:58 2008''

Revision as of 07:01, 1 July 2008

File:1hwr.png

Template:STRUCTURE 1hwr

MOLECULAR RECOGNITION OF CYCLIC UREA HIV PROTEASE INHIBITORS

Template:ABSTRACT PUBMED 9575185

About this Structure

1HWR is a Single protein structure of sequence from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.

Reference

Molecular recognition of cyclic urea HIV-1 protease inhibitors., Ala PJ, DeLoskey RJ, Huston EE, Jadhav PK, Lam PY, Eyermann CJ, Hodge CN, Schadt MC, Lewandowski FA, Weber PC, McCabe DD, Duke JL, Chang CH, J Biol Chem. 1998 May 15;273(20):12325-31. PMID:9575185

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