1i42: Difference between revisions

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[[Image:1i42.gif|left|200px]]
{{Seed}}
[[Image:1i42.png|left|200px]]


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{{STRUCTURE_1i42|  PDB=1i42  |  SCENE=  }}  
{{STRUCTURE_1i42|  PDB=1i42  |  SCENE=  }}  


'''NMR STRUCTURE OF THE UBX DOMAIN FROM P47'''
===NMR STRUCTURE OF THE UBX DOMAIN FROM P47===




==Overview==
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p47 is the major protein identified in complex with the cytosolic AAA ATPase p97. It functions as an essential cofactor of p97-regulated membrane fusion, which has been suggested to disassemble t-t-SNARE complexes and prepare them for further rounds of membrane fusion. Here, we report the high-resolution NMR structure of the C-terminal domain from p47. It comprises a UBX domain and a 13 residue long structured N-terminal extension. The UBX domain adopts a characteristic ubiquitin fold with a betabetaalphabetabetaalphabeta secondary structure arrangement. Three hydrophobic residues from the N-terminal extension pack closely against a cleft in the UBX domain. We also identify, for the first time, the p97 interaction surface using NMR chemical shift perturbation studies.
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{{ABSTRACT_PUBMED_11478859}}


==About this Structure==
==About this Structure==
1I42 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I42 OCA].  
1I42 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I42 OCA].  


==Reference==
==Reference==
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[[Category: Ubx]]
[[Category: Ubx]]
[[Category: Unusual n-terminal feature]]
[[Category: Unusual n-terminal feature]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:32:46 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:23:04 2008''

Revision as of 07:23, 1 July 2008

File:1i42.png

Template:STRUCTURE 1i42

NMR STRUCTURE OF THE UBX DOMAIN FROM P47

Template:ABSTRACT PUBMED 11478859

About this Structure

1I42 is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Solution structure and interaction surface of the C-terminal domain from p47: a major p97-cofactor involved in SNARE disassembly., Yuan X, Shaw A, Zhang X, Kondo H, Lally J, Freemont PS, Matthews S, J Mol Biol. 2001 Aug 10;311(2):255-63. PMID:11478859

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