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| [[Image:1i9v.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1i9v| PDB=1i9v | SCENE= }} | | {{STRUCTURE_1i9v| PDB=1i9v | SCENE= }} |
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| '''CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX'''
| | ===CRYSTAL STRUCTURE ANALYSIS OF A TRNA-NEOMYCIN COMPLEX=== |
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| ==Overview==
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| Aminoglycosides bind to RNA and interfere with its function, and it has been suggested that aminoglycoside binding to RNA displaces essential divalent metal ions. Here we demonstrate that addition of various aminoglycosides inhibited Pb2+-induced cleavage of yeast tRNA(Phe). Cocrystallization of yeast tRNA(Phe) and an aminoglycoside, neomycin B, resulted in crystals that diffracted to 2.6 A and the structure of the complex was solved by molecular replacement. The structure shows that the neomycin B binding site overlaps with known divalent metal ion binding sites in yeast tRNA(Phe), providing direct evidence for the hypothesis that aminoglycosides displace metal ions. Additionally, the neomycin B binding site overlaps with major determinants for Escherichia coli phenylalanyl-tRNA-synthetase. Here we present data demonstrating that addition of neomycin B inhibited aminoacylation of E. coli tRNA(Phe) in the mid microM range. Given that aminoglycoside and metal ion binding sites overlap, we discuss that aminoglycosides can be considered as 'metal mimics'.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11373618}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11373618 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11373618}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Transfer rna]] | | [[Category: Transfer rna]] |
| [[Category: Yeast]] | | [[Category: Yeast]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:45:03 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:38:33 2008'' |
