1ifw: Difference between revisions

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[[Image:1ifw.gif|left|200px]]
{{Seed}}
[[Image:1ifw.png|left|200px]]


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{{STRUCTURE_1ifw|  PDB=1ifw  |  SCENE=  }}  
{{STRUCTURE_1ifw|  PDB=1ifw  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF C-TERMINAL DOMAIN OF POLY(A) BINDING PROTEIN FROM SACCHAROMYCES CEREVISIAE'''
===SOLUTION STRUCTURE OF C-TERMINAL DOMAIN OF POLY(A) BINDING PROTEIN FROM SACCHAROMYCES CEREVISIAE===




==Overview==
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We have determined the solution structure of the PABC domain from Saccharomyces cerevisiae Pab1p and mapped its peptide-binding site. PABC domains are peptide binding domains found in poly(A)-binding proteins (PABP) and are a subset of HECT-family E3 ubiquitin ligases (also known as hyperplastic discs proteins (HYDs)). In mammals, the PABC domain of PABP functions to recruit several different translation factors to the mRNA poly(A) tail. PABC domains are highly conserved, with high specificity for peptide sequences of roughly 12 residues with conserved alanine, phenylalanine, and proline residues at positions 7, 10, and 12. Compared with human PABP, the yeast PABC domain is missing the first alpha helix, contains two extra amino acids between helices 2 and 3, and has a strongly bent C-terminal helix. These give rise to unique peptide binding specificity wherein yeast PABC binds peptides from Paip2 and RF3 but not Paip1. Mapping of the peptide-binding site reveals that the bend in the C-terminal helix disrupts binding interactions with the N terminus of peptide ligands and leads to greatly reduced binding affinity for the peptides tested. No high affinity or natural binding partners from S. cerevisiae could be identified by sequence analysis of known PABC ligands. Comparison of the three known PABC structures shows that the features responsible for peptide binding are highly conserved and responsible for the distinct but overlapping binding specificities.
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{{ABSTRACT_PUBMED_11940585}}


==About this Structure==
==About this Structure==
1IFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IFW OCA].  
1IFW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IFW OCA].  


==Reference==
==Reference==
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[[Category: Sprules, T.]]
[[Category: Sprules, T.]]
[[Category: All-helical domain]]
[[Category: All-helical domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:57:26 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:54:44 2008''

Revision as of 07:54, 1 July 2008

File:1ifw.png

Template:STRUCTURE 1ifw

SOLUTION STRUCTURE OF C-TERMINAL DOMAIN OF POLY(A) BINDING PROTEIN FROM SACCHAROMYCES CEREVISIAE

Template:ABSTRACT PUBMED 11940585

About this Structure

1IFW is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Solution structure of the orphan PABC domain from Saccharomyces cerevisiae poly(A)-binding protein., Kozlov G, Siddiqui N, Coillet-Matillon S, Trempe JF, Ekiel I, Sprules T, Gehring K, J Biol Chem. 2002 Jun 21;277(25):22822-8. Epub 2002 Apr 8. PMID:11940585

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