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| [[Image:1ig5.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1ig5| PDB=1ig5 | SCENE= }} | | {{STRUCTURE_1ig5| PDB=1ig5 | SCENE= }} |
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| '''BOVINE CALBINDIN D9K BINDING MG2+'''
| | ===BOVINE CALBINDIN D9K BINDING MG2+=== |
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| ==Overview==
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| The three-dimensional structures of the magnesium- and manganese-bound forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly identical but deviate significantly from both the calcium bound form and the metal ion-free (apo) form. The largest structural differences are seen in the C-terminal EF-hand, and involve changes in both metal ion coordination and helix packing. The N-terminal calcium binding site is not occupied by any metal ion in the magnesium and manganese structures, and shows little structural deviation from the apo and calcium bound forms. 1H-NMR and UV spectroscopic studies at physiological ion concentrations show that the C-terminal site of the protein is significantly populated by magnesium at resting cell calcium levels, and that there is a negative allosteric interaction between magnesium and calcium binding. Calcium binding was found to occur with positive cooperativity at physiological magnesium concentration. | | The line below this paragraph, {{ABSTRACT_PUBMED_9194174}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 9194174 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_9194174}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Ef-hand]] | | [[Category: Ef-hand]] |
| [[Category: Magnesium binding]] | | [[Category: Magnesium binding]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:57:50 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:56:35 2008'' |
