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| {{STRUCTURE_1ihj| PDB=1ihj | SCENE= }} | | {{STRUCTURE_1ihj| PDB=1ihj | SCENE= }} |
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| '''Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide'''
| | ===Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide=== |
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| ==Overview==
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| In Drosophila, phototransduction is mediated by G(q)-activation of phospholipase C and is a well studied model system for understanding the kinetics of signal initiation, propagation and termination controlled by G proteins. The proper intracellular targeting and spatial arrangement of most proteins involved in fly phototransduction require the multi-domain scaffolding protein InaD, composed almost entirely of five PDZ domains, which independently bind various proteins including NorpA, the relevant phospho lipase C-beta isozyme. We have determined the crystal structure of the N-terminal PDZ domain of InaD bound to a peptide corresponding to the C-terminus of NorpA to 1.8 A resolution. The structure highlights an intermolecular disulfide bond necessary for high affinity interaction as determined by both in vitro and in vivo studies. Since other proteins also possess similar, cysteine-containing consensus sequences for binding PDZ domains, this disulfide-mediated 'dock-and-lock' interaction of PDZ domains with their ligands may be a relatively ubiquitous mode of coordinating signaling pathways.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11500369}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11500369 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11500369}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Intermolecular disulfide bond]] | | [[Category: Intermolecular disulfide bond]] |
| [[Category: Pdz domain]] | | [[Category: Pdz domain]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:00:18 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 12:11:46 2008'' |
