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| {{STRUCTURE_1inn| PDB=1inn | SCENE= }} | | {{STRUCTURE_1inn| PDB=1inn | SCENE= }} |
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| '''CRYSTAL STRUCTURE OF D. RADIODURANS LUXS, P21'''
| | ===CRYSTAL STRUCTURE OF D. RADIODURANS LUXS, P21=== |
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| ==Overview==
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| BACKGROUND: Quorum sensing is the mechanism by which bacteria control gene expression in response to cell density. Two major quorum-sensing systems have been identified, system 1 and system 2, each with a characteristic signaling molecule (autoinducer-1, or AI-1, in the case of system 1, and AI-2 in system 2). The luxS gene is required for the AI-2 system of quorum sensing. LuxS and AI-2 have been described in both Gram-negative and Gram-positive bacterial species and have been shown to be involved in the expression of virulence genes in several pathogens. RESULTS: The structure of the LuxS protein from three different bacterial species with resolutions ranging from 1.8 A to 2.4 A has been solved using an X-ray crystallographic structural genomics approach. The structure of LuxS reported here is seen to have a new alpha-beta fold. In all structures, an equivalent homodimer is observed. A metal ion identified as zinc was seen bound to a Cys-His-His triad. Methionine was found bound to the protein near the metal and at the dimer interface. CONCLUSIONS: These structures provide support for a hypothesis that explains the in vivo action of LuxS. Specifically, acting as a homodimer, the protein binds a methionine analog, S-ribosylhomocysteine (SRH). The zinc atom is in position to cleave the ribose ring in a step along the synthesis pathway of AI-2.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11435117}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11435117 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11435117}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Sanderson, W E.]] | | [[Category: Sanderson, W E.]] |
| [[Category: Alpha-beta fold]] | | [[Category: Alpha-beta fold]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:11:34 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:36:57 2008'' |
