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[[Image:1ire.gif|left|200px]]
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[[Image:1ire.png|left|200px]]


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{{STRUCTURE_1ire|  PDB=1ire  |  SCENE=  }}  
{{STRUCTURE_1ire|  PDB=1ire  |  SCENE=  }}  


'''Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila'''
===Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila===




==Overview==
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The crystal structure of cobalt-containing nitrile hydratase from Pseudonocardia thermophila JCM 3095 at 1.8 A resolution revealed the structure of the noncorrin cobalt at the catalytic center. Two cysteine residues (alphaCys(111) and alphaCys(113)) coordinated to the cobalt were posttranslationally modified to cysteine-sulfinic acid and to cysteine-sulfenic acid, respectively, like in iron-containing nitrile hydratase. A tryptophan residue (betaTrp(72)), which may be involved in substrate binding, replaced the tyrosine residue of iron-containing nitrile hydratase. The difference seems to be responsible for the preference for aromatic nitriles rather than aliphatic ones of cobalt-containing nitrile hydratase.
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{{ABSTRACT_PUBMED_11700034}}


==About this Structure==
==About this Structure==
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[[Category: Non-corrin cobalt]]
[[Category: Non-corrin cobalt]]
[[Category: Post-translational modification]]
[[Category: Post-translational modification]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:19:18 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:51:53 2008''

Revision as of 10:51, 1 July 2008

File:1ire.png

Template:STRUCTURE 1ire

Crystal Structure of Co-type nitrile hydratase from Pseudonocardia thermophila

Template:ABSTRACT PUBMED 11700034

About this Structure

1IRE is a Protein complex structure of sequences from Pseudonocardia thermophila. Full crystallographic information is available from OCA.

Reference

Crystal structure of cobalt-containing nitrile hydratase., Miyanaga A, Fushinobu S, Ito K, Wakagi T, Biochem Biophys Res Commun. 2001 Nov 16;288(5):1169-74. PMID:11700034

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