1it7: Difference between revisions

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[[Image:1it7.gif|left|200px]]
{{Seed}}
[[Image:1it7.png|left|200px]]


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{{STRUCTURE_1it7|  PDB=1it7  |  SCENE=  }}  
{{STRUCTURE_1it7|  PDB=1it7  |  SCENE=  }}  


'''Crystal structure of archaeosine tRNA-guanine transglycosylase complexed with guanine'''
===Crystal structure of archaeosine tRNA-guanine transglycosylase complexed with guanine===




==Overview==
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Archaeosine tRNA-guanine transglycosylase (ArcTGT) catalyzes the exchange of guanine at position 15 in the D-loop of archaeal tRNAs with a free 7-cyano-7-deazaguanine (preQ(0)) base, as the first step in the biosynthesis of an archaea-specific modified base, archaeosine (7-formamidino-7-deazaguanosine). We determined the crystal structures of ArcTGT from Pyrococcus horikoshii at 2.2 A resolution and its complexes with guanine and preQ(0), at 2.3 and 2.5 A resolutions, respectively. The N-terminal catalytic domain folds into an (alpha/beta)(8) barrel with a characteristic zinc-binding site, showing structural similarity with that of the bacterial queuosine TGT (QueTGT), which is involved in queuosine (7-[[(4,5-cis-dihydroxy-2-cyclopenten-1-yl)-amino]methyl]-7-deazaguanosine ) biosynthesis and targets the tRNA anticodon. ArcTGT forms a dimer, involving the zinc-binding site and the ArcTGT-specific C-terminal domain. The C-terminal domains have novel folds, including an OB fold-like "PUA domain", whose sequence is widely conserved in eukaryotic and archaeal RNA modification enzymes. Therefore, the C-terminal domains may be involved in tRNA recognition. In the free-form structure of ArcTGT, an alpha-helix located at the rim of the (alpha/beta)(8) barrel structure is completely disordered, while it is ordered in the guanine-bound and preQ(0)-bound forms. Structural comparison of the ArcTGT.preQ(0), ArcTGT.guanine, and QueTGT.preQ(1) complexes provides novel insights into the substrate recognition mechanisms of ArcTGT.
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{{ABSTRACT_PUBMED_12054814}}


==About this Structure==
==About this Structure==
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[[Category: Rsgi]]
[[Category: Rsgi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:23:14 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:57:44 2008''

Revision as of 10:57, 1 July 2008

File:1it7.png

Template:STRUCTURE 1it7

Crystal structure of archaeosine tRNA-guanine transglycosylase complexed with guanine

Template:ABSTRACT PUBMED 12054814

About this Structure

1IT7 is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

Reference

Crystal structure of archaeosine tRNA-guanine transglycosylase., Ishitani R, Nureki O, Fukai S, Kijimoto T, Nameki N, Watanabe M, Kondo H, Sekine M, Okada N, Nishimura S, Yokoyama S, J Mol Biol. 2002 May 3;318(3):665-77. PMID:12054814

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