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| {{STRUCTURE_1iw4| PDB=1iw4 | SCENE= }} | | {{STRUCTURE_1iw4| PDB=1iw4 | SCENE= }} |
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| '''Solution structure of ascidian trypsin inhibitor'''
| | ===Solution structure of ascidian trypsin inhibitor=== |
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| ==Overview==
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| The three-dimensional solution structure of ascidian trypsin inhibitor (ATI), a 55 amino acid residue protein with four disulfide bridges, was determined by means of two-dimensional nuclear magnetic resonance (2D NMR) spectroscopy. The resulting structure of ATI was characterized by an alpha-helical conformation in residues 35-42 and a three-stranded antiparallel beta-sheet in residues 22-26, 29-32, and 48-50. The presence of an alpha-helical conformation was predicted from the consensus sequences of the cystine-stabilized alpha-helical (CSH) motif, which is characterized by an alpha-helix structure in the Cys-X(1)-X(2)-X(3)-Cys portion (corresponding to residues 37-41), linking to the Cys-X-Cys portion (corresponding to residues 12-14) folded in an extended structure. The secondary structure and the overall folding of the main chain of ATI were very similar to those of the Kazal-type inhibitors, such as Japanese quail ovomucoid third domain (OMJPQ3) and leech-derived tryptase inhibitor form C (LDTI-C), although ATI does not show extensive sequence homology to these inhibitors except for a few amino acid residues and six of eight half-cystines. On the basis of these findings, we realign the amino acid sequences of representative Kazal-type inhibitors including ATI and discuss the unique structure of ATI with four disulfide bridges.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12186551}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12186551 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12186551}} |
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| ==About this Structure== | | ==About this Structure== |
| 1IW4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halocynthia_roretzi Halocynthia roretzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IW4 OCA]. | | 1IW4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halocynthia_roretzi Halocynthia roretzi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IW4 OCA]. |
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| ==Reference== | | ==Reference== |
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| [[Category: Solution structure]] | | [[Category: Solution structure]] |
| [[Category: Trypsin inhibitor]] | | [[Category: Trypsin inhibitor]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:29:40 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:06:30 2008'' |
