1iym: Difference between revisions

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[[Image:1iym.jpg|left|200px]]
{{Seed}}
[[Image:1iym.png|left|200px]]


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{{STRUCTURE_1iym|  PDB=1iym  |  SCENE=  }}  
{{STRUCTURE_1iym|  PDB=1iym  |  SCENE=  }}  


'''RING-H2 finger domain of EL5'''
===RING-H2 finger domain of EL5===




==Overview==
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EL5, a RING-H2 finger protein, is rapidly induced by N-acetylchitooligosaccharides in rice cell. We expressed the EL5 RING-H2 finger domain in Escherichia coli and determined its structure in solution by NMR spectroscopy. The EL5 RING-H2 finger domain consists of two-stranded beta-sheets (beta1, Ala(147)-Phe(149); beta2, Gly(156)-His(158)), one alpha-helix (Cys(161)-Leu(166)), and two large N- and C-terminal loops. It is stabilized by two tetrahedrally coordinated zinc ions. This structure is similar to that of other RING finger domains of proteins of known function. From structural analogies, we inferred that the EL5 RING-H2 finger is a binding domain for ubiquitin-conjugating enzyme (E2). The binding site is probably formed by solvent-exposed hydrophobic residues of the N- and C-terminal loops and the alpha-helix. We demonstrated that the fusion protein with EL5-(96-181) and maltose-binding protein (MBP) was polyubiquitinated by incubation with ubiquitin, ubiquitin-activating enzyme (E1), and a rice E2 protein, OsUBC5b. This supported the idea that the EL5 RING finger domain is essential for ubiquitin-ligase activity of EL5. By NMR titration experiments, we identified residues that are critical for the interaction between the EL5 RING-H2 finger and OsUBC5b. We conclude that the RING-H2 finger domain of EL5 is the E2 binding site of EL5.
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{{ABSTRACT_PUBMED_12588869}}


==About this Structure==
==About this Structure==
1IYM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IYM OCA].  
1IYM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IYM OCA].  


==Reference==
==Reference==
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[[Category: Ring-h2 finger]]
[[Category: Ring-h2 finger]]
[[Category: Ubiquitin ligase]]
[[Category: Ubiquitin ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:13:48 2008''

Revision as of 11:13, 1 July 2008

File:1iym.png

Template:STRUCTURE 1iym

RING-H2 finger domain of EL5

Template:ABSTRACT PUBMED 12588869

About this Structure

1IYM is a Single protein structure of sequence from Oryza sativa. Full experimental information is available from OCA.

Reference

High precision NMR structure and function of the RING-H2 finger domain of EL5, a rice protein whose expression is increased upon exposure to pathogen-derived oligosaccharides., Katoh S, Hong C, Tsunoda Y, Murata K, Takai R, Minami E, Yamazaki T, Katoh E, J Biol Chem. 2003 Apr 25;278(17):15341-8. Epub 2003 Feb 14. PMID:12588869

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