1j5k: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
Line 1: Line 1:
[[Image:1j5k.gif|left|200px]]
{{Seed}}
[[Image:1j5k.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1j5k|  PDB=1j5k  |  SCENE=  }}  
{{STRUCTURE_1j5k|  PDB=1j5k  |  SCENE=  }}  


'''COMPLEX OF THE KH3 DOMAIN OF HNRNP K WITH A SINGLE_STRANDED 10MER DNA OLIGONUCLEOTIDE'''
===COMPLEX OF THE KH3 DOMAIN OF HNRNP K WITH A SINGLE_STRANDED 10MER DNA OLIGONUCLEOTIDE===




==Overview==
<!--  
To elucidate the basis of sequence-specific single-stranded (ss) DNA recognition by K homology (KH) domains, we have solved the solution structure of a complex between the KH3 domain of the transcriptional regulator heterogeneous nuclear ribonucleoprotein K (hnRNP K) and a 10mer ssDNA. We show that hnRNP K KH3 specifically recognizes a tetrad of sequence 5'd-TCCC. The complex is stabilized by a dense network of methyl-oxygen hydrogen bonds involving the methyl groups of three isoleucine residues and the O2 and N3 atoms of the two central cytosine bases. Comparison with the recently solved structure of a specific protein-ssDNA complex involving the KH3 and KH4 domains of the far upstream element (FUSE) binding protein FBP suggests that the amino acid located five residues N-terminal of the invariant GXXG motif, which is characteristic of all KH domains, plays a crucial role in discrimination of the first two bases of the tetrad.
The line below this paragraph, {{ABSTRACT_PUBMED_12093748}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12093748 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_12093748}}


==About this Structure==
==About this Structure==
1J5K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5K OCA].  
1J5K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J5K OCA].  


==Reference==
==Reference==
Line 29: Line 33:
[[Category: Single-stranded dna binding protein]]
[[Category: Single-stranded dna binding protein]]
[[Category: Transcription factor]]
[[Category: Transcription factor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:49:54 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 14:32:06 2008''

Revision as of 11:32, 1 July 2008

File:1j5k.png

Template:STRUCTURE 1j5k

COMPLEX OF THE KH3 DOMAIN OF HNRNP K WITH A SINGLE_STRANDED 10MER DNA OLIGONUCLEOTIDE

Template:ABSTRACT PUBMED 12093748

About this Structure

1J5K is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Molecular basis of sequence-specific single-stranded DNA recognition by KH domains: solution structure of a complex between hnRNP K KH3 and single-stranded DNA., Braddock DT, Baber JL, Levens D, Clore GM, EMBO J. 2002 Jul 1;21(13):3476-85. PMID:12093748

Page seeded by OCA on Tue Jul 1 14:32:06 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1j5k&oldid=615043"