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| [[Image:1jb1.gif|left|200px]] | | {{Seed}} |
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| {{STRUCTURE_1jb1| PDB=1jb1 | SCENE= }} | | {{STRUCTURE_1jb1| PDB=1jb1 | SCENE= }} |
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| '''Lactobacillus casei HprK/P Bound to Phosphate'''
| | ===Lactobacillus casei HprK/P Bound to Phosphate=== |
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| ==Overview==
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| HPr kinase/phosphatase (HprK/P) is a key regulatory enzyme controlling carbon metabolism in Gram- positive bacteria. It catalyses the ATP-dependent phosphorylation of Ser46 in HPr, a protein of the phosphotransferase system, and also its dephosphorylation. HprK/P is unrelated to eukaryotic protein kinases, but contains the Walker motif A characteristic of nucleotide-binding proteins. We report here the X-ray structure of an active fragment of Lactobacillus casei HprK/P at 2.8 A resolution, solved by the multiwavelength anomalous dispersion method on a seleniated protein (PDB code 1jb1). The protein is a hexamer, with each subunit containing an ATP-binding domain similar to nucleoside/nucleotide kinases, and a putative HPr-binding domain unrelated to the substrate-binding domains of other kinases. The Walker motif A forms a typical P-loop which binds inorganic phosphate in the crystal. We modelled ATP binding by comparison with adenylate kinase, and designed a tentative model of the complex with HPr based on a docking simulation. The results confirm that HprK/P represents a new family of protein kinases, first identified in bacteria, but which may also have members in eukaryotes.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11483495}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11483495 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11483495}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: P-loop]] | | [[Category: P-loop]] |
| [[Category: Protein kinase]] | | [[Category: Protein kinase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:00:04 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 19:56:45 2008'' |
