1ji9: Difference between revisions

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[[Image:1ji9.jpg|left|200px]]
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[[Image:1ji9.png|left|200px]]


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{{STRUCTURE_1ji9|  PDB=1ji9  |  SCENE=  }}  
{{STRUCTURE_1ji9|  PDB=1ji9  |  SCENE=  }}  


'''Solution structure of the alpha-domain of mouse metallothionein-3'''
===Solution structure of the alpha-domain of mouse metallothionein-3===




==Overview==
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The brain specific member of the metallothionein (MT) family of proteins, metallothionein-3, inhibits the growth and survival of neurons, in contrast to the ubiquitous mammalian MT isoforms, MT-1 and MT-2, that are found in most tissues and are thought to function in metal ion homeostasis and detoxification. Solution NMR was utilized to determine the structural and dynamic differences of MT-3 from MT-1 and 2. The high-resolution solution structure of the C-terminal alpha-domain of recombinant mouse MT-3 revealed a tertiary fold very similar to MT-1 and 2, except for a loop that accommodates an acidic insertion relative to these isoforms. This loop was distinguished from the rest of the domain by dynamics of the backbone on the nano- to picosecond time-scale shown by (15)N relaxation studies and was identified as a possible interaction site with other proteins. The N-terminal beta-domain contains the region responsible for the growth inhibitory activity, a CPCP tetrapeptide close to the N-terminus. Because of exchange broadening of a large number of the NMR signals from this domain, homology modeling was utilized to calculate models for the beta-domain and suggested that while the backbone fold of the MT-3 beta-domain is identical to MT-1 and 2, the second proline responsible for the activity, Pro9, may show structural heterogeneity. (15)N relaxation analyses implied fast internal motions for the beta-domain. On the basis of these observations, we conclude that the growth inhibitory activity exhibited by MT-3 is a result of a combination of local structural differences and global dynamics in the beta-domain.
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{{ABSTRACT_PUBMED_11560491}}


==About this Structure==
==About this Structure==
1JI9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI9 OCA].  
1JI9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI9 OCA].  


==Reference==
==Reference==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:17:15 2008''

Revision as of 17:17, 1 July 2008

File:1ji9.png

Template:STRUCTURE 1ji9

Solution structure of the alpha-domain of mouse metallothionein-3

Template:ABSTRACT PUBMED 11560491

About this Structure

1JI9 is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3., Oz G, Zangger K, Armitage IM, Biochemistry. 2001 Sep 25;40(38):11433-41. PMID:11560491

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