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[[Image:1jjd.gif|left|200px]]
{{Seed}}
[[Image:1jjd.png|left|200px]]


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{{STRUCTURE_1jjd|  PDB=1jjd  |  SCENE=  }}  
{{STRUCTURE_1jjd|  PDB=1jjd  |  SCENE=  }}  


'''NMR structure of the Cyanobacterial Metallothionein SmtA'''
===NMR structure of the Cyanobacterial Metallothionein SmtA===




==Overview==
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Zinc is essential for many cellular processes, including DNA synthesis, transcription, and translation, but excess can be toxic. A zinc-induced gene, smtA, is required for normal zinc-tolerance in the cyanobacterium Synechococcus PCC 7942. Here we report that the protein SmtA contains a cleft lined with Cys-sulfur and His-imidazole ligands that binds four zinc ions in a Zn(4)Cys(9)His(2) cluster. The thiolate sulfurs of five Cys ligands provide bridges between the two ZnCys(4) and two ZnCys(3)His sites, giving two fused six-membered rings with distorted boat conformations. The inorganic core strongly resembles the Zn(4)Cys(11) cluster of mammalian metallothionein, despite different amino acid sequences, a different linear order of the ligands, and presence of histidine ligands. Also, SmtA contains elements of secondary structure not found in metallothioneins. One of the two Cys(4)-coordinated zinc ions in SmtA readily exchanges with exogenous metal ((111)Cd), whereas the other is inert. The thiolate sulfur ligands bound to zinc in this site are buried within the protein. Regions of beta-strand and alpha-helix surround the inert site to form a zinc finger resembling the zinc fingers in GATA and LIM-domain proteins. Eukaryotic zinc fingers interact specifically with other proteins or DNA and an analogous interaction can therefore be anticipated for prokaryotic zinc fingers. SmtA now provides structural proof for the existence of zinc fingers in prokaryotes, and sequences related to the zinc finger motif can be identified in several bacterial genomes.
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{{ABSTRACT_PUBMED_11493688}}


==About this Structure==
==About this Structure==
1JJD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJD OCA].  
1JJD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJD OCA].  


==Reference==
==Reference==
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[[Category: Zinc cluster]]
[[Category: Zinc cluster]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:17:42 2008''
 
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Revision as of 17:20, 1 July 2008

File:1jjd.png

Template:STRUCTURE 1jjd

NMR structure of the Cyanobacterial Metallothionein SmtA

Template:ABSTRACT PUBMED 11493688

About this Structure

1JJD is a Single protein structure of sequence from Synechococcus sp.. Full experimental information is available from OCA.

Reference

A metallothionein containing a zinc finger within a four-metal cluster protects a bacterium from zinc toxicity., Blindauer CA, Harrison MD, Parkinson JA, Robinson AK, Cavet JS, Robinson NJ, Sadler PJ, Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9593-8. Epub 2001 Aug 7. PMID:11493688

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