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[[Image:1jp0.gif|left|200px]]
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[[Image:1jp0.png|left|200px]]


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{{STRUCTURE_1jp0|  PDB=1jp0  |  SCENE=  }}  
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'''NMR Structure of the LP5.1 Hairpin from Bacillus RNase P RNA Refined WITHOUT Residual Dipolar Couplings'''
===NMR Structure of the LP5.1 Hairpin from Bacillus RNase P RNA Refined WITHOUT Residual Dipolar Couplings===




==Overview==
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Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA are thought to form a structural truss to support RNA folding and activity. We determined the structure of this element by NMR and refined the structure using residual dipolar couplings from a sample weakly oriented in a dilute liquid crystalline mixture of polyethylene glycol and hexanol. Dipolar coupling refinement improved the global precision of the structure from 1.5 to 1.2 A (to the mean), revised the bend angle between segments of the P5.1 stem and corroborated the structure of the loop region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on opposite sides of the loop, distinguishing it from GNRA tetraloops. The unusual conformation of the juxtaposed uracil residues within the hexaloop may explain their requirement in transactivation assays.
The line below this paragraph, {{ABSTRACT_PUBMED_11927952}}, adds the Publication Abstract to the page
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{{ABSTRACT_PUBMED_11927952}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JP0 OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JP0 OCA].  


==Reference==
==Reference==
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[[Category: Rnase p]]
[[Category: Rnase p]]
[[Category: Ugnrau]]
[[Category: Ugnrau]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:32:04 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:34:46 2008''

Revision as of 17:34, 1 July 2008

File:1jp0.png

Template:STRUCTURE 1jp0

NMR Structure of the LP5.1 Hairpin from Bacillus RNase P RNA Refined WITHOUT Residual Dipolar Couplings

Template:ABSTRACT PUBMED 11927952

About this Structure

Full experimental information is available from OCA.

Reference

Structure of the UGAGAU hexaloop that braces Bacillus RNase P for action., Leeper TC, Martin MB, Kim H, Cox S, Semenchenko V, Schmidt FJ, Van Doren SR, Nat Struct Biol. 2002 May;9(5):397-403. PMID:11927952

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