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[[Image:1jth.gif|left|200px]]
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[[Image:1jth.png|left|200px]]


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{{STRUCTURE_1jth|  PDB=1jth  |  SCENE=  }}  
{{STRUCTURE_1jth|  PDB=1jth  |  SCENE=  }}  


'''Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a'''
===Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a===




==Overview==
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SNARE proteins are required for intracellular membrane fusion. In the neuron, the plasma membrane SNAREs syntaxin 1a and SNAP25 bind to VAMP2 found on neurotransmitter-containing vesicles. These three proteins contain "SNARE regions" that mediate their association into stable tetrameric coiled-coil structures. Syntaxin 1a contributes one such region, designated H3, and SNAP25 contributes two SNARE regions to the fusogenic complex with VAMP2. Syntaxin 1a H3 (syn1aH3) and SNAP25 can form a stable assembly, which can then be bound by VAMP2 to form the full SNARE complex. Here we show that syn1aH3 can also form a stable but kinetically trapped complex with the N-terminal SNARE region of SNAP25 (S25N). The crystal structure of this complex reveals an extended parallel four-helix bundle similar to that of the core SNARE and the syn1aH3-SNAP25 complexes. The inherent ability of syn1aH3 and S25N to associate stably in vitro implies that the intracellular fusion machinery must prevent formation of, or remove, any non-productive complexes. Comparison with the syn1aH3-SNAP25 complex suggests that the linkage of the N- and C-terminal SNAP25 SNARE regions is kinetically advantageous in preventing formation of the non-productive syn1aH3-S25N complex. We also demonstrate that the syn1aH3-S25N complex can be disassembled by alpha-SNAP and N-ethylmaleimide-sensitive factor.
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{{ABSTRACT_PUBMED_11533035}}


==About this Structure==
==About this Structure==
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[[Category: Coiled-coil]]
[[Category: Coiled-coil]]
[[Category: Polar layer]]
[[Category: Polar layer]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:53:48 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:46:34 2008''

Revision as of 17:46, 1 July 2008

File:1jth.png

Template:STRUCTURE 1jth

Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a

Template:ABSTRACT PUBMED 11533035

About this Structure

1JTH is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Crystal structure and biophysical properties of a complex between the N-terminal SNARE region of SNAP25 and syntaxin 1a., Misura KM, Gonzalez LC Jr, May AP, Scheller RH, Weis WI, J Biol Chem. 2001 Nov 2;276(44):41301-9. Epub 2001 Aug 30. PMID:11533035

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