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| {{STRUCTURE_1jto| PDB=1jto | SCENE= }} | | {{STRUCTURE_1jto| PDB=1jto | SCENE= }} |
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| '''Degenerate interfaces in antigen-antibody complexes'''
| | ===Degenerate interfaces in antigen-antibody complexes=== |
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| ==Overview==
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| In most of the work dealing with the analysis of protein-protein interfaces, a single X-ray structure is available or selected, and implicitly it is assumed that this structure corresponds to the optimal complex for this pair of proteins. However, we have found a degenerate interface in a high-affinity antibody-antigen complex: the two independent complexes of the camel variable domain antibody fragment cAb-Lys3 and its antigen hen egg white lysozyme present in the asymmetric unit of our crystals show a difference in relative orientation between antibody and antigen, leading to important differences at the protein-protein interface. A third cAb-Lys3-hen lysozyme complex in a different crystal form adopts yet another relative orientation. Our results show that protein-protein interface characteristics can vary significantly between different specimens of the same high-affinity antibody-protein antigen complex. Consideration should be given to this type of observation when trying to establish general protein-protein interface characteristics.
| | The line below this paragraph, {{ABSTRACT_PUBMED_11676532}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 11676532 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_11676532}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Interface]] | | [[Category: Interface]] |
| [[Category: Vhh]] | | [[Category: Vhh]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:54:27 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 20:47:24 2008'' |
