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| [[Image:1jvy.gif|left|200px]] | | {{Seed}} |
| | [[Image:1jvy.png|left|200px]] |
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| {{STRUCTURE_1jvy| PDB=1jvy | SCENE= }} | | {{STRUCTURE_1jvy| PDB=1jvy | SCENE= }} |
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| '''Maltodextrin-binding protein variant D207C/A301GS/P316C with beta-mercaptoethanol mixed disulfides'''
| | ===Maltodextrin-binding protein variant D207C/A301GS/P316C with beta-mercaptoethanol mixed disulfides=== |
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| ==Overview==
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| Cysteine substitutions were engineered on the surface of maltose binding protein to produce crystine fibers, linear polymers of folded protein formed within a crystal. Disulfide bond formation between adjacent protein molecules within the lattice was monitored by X-ray crystallography. The cross-linked crystals were resistant to dissolution in water or neutral buffer solutions, even though the cross-linking was one-dimensional. However, crystine fibers were observed by transmission electron microscopy to dissociate from the crystals in acidic solutions. Some fibers remained associated as two-dimensional bundles or sheets, with a repeat unit along the fibers consistent with the packing of the individual protein molecules in the crystal. Neutralization of the acidic solutions caused the fibers to re-associate as a solid. Crystine threads were drawn out of this solution. In scanning electron microscopy images, many individual fibers could be seen unwinding from the ends of some threads. Crystine fibers are a new type of biomolecular material with potential applications wherever the use of proteins in a fibrous form is desirable, for example, the incorporation of enzymes into cloth or filtration material.
| | The line below this paragraph, {{ABSTRACT_PUBMED_12538909}}, adds the Publication Abstract to the page |
| | (as it appears on PubMed at http://www.pubmed.gov), where 12538909 is the PubMed ID number. |
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| | {{ABSTRACT_PUBMED_12538909}} |
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| ==About this Structure== | | ==About this Structure== |
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| [[Category: Disulfide]] | | [[Category: Disulfide]] |
| [[Category: Intermolecular]] | | [[Category: Intermolecular]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:59:34 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 21:02:42 2008'' |
