1k43: Difference between revisions

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[[Image:1k43.gif|left|200px]]
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[[Image:1k43.png|left|200px]]


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{{STRUCTURE_1k43|  PDB=1k43  |  SCENE=  }}  
{{STRUCTURE_1k43|  PDB=1k43  |  SCENE=  }}  


'''10 Structure Ensemble of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)'''
===10 Structure Ensemble of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)===




==Overview==
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Here we present a combinatorial approach to evolve a stable beta-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a beta-hairpin structure population of around 30%, we selected four positions to build up a combinatorial library of 20(4) sequences. Deconvolution of the library using circular dichroism reduced such a sequence complexity to 36 defined sequences. Circular dichroism and NMR of these peptides resulted in the identification of two linear 14-aa-long peptides that in plain buffered solutions showed a percentage of beta-hairpin structure higher than 70%. Our results show how combinatorial approaches can be used to obtain highly structured peptide sequences that could be used as templates in which functionality can be introduced.
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{{ABSTRACT_PUBMED_11782528}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K43 OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K43 OCA].  


==Reference==
==Reference==
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[[Category: Serrano, L.]]
[[Category: Serrano, L.]]
[[Category: Beta-hairpin]]
[[Category: Beta-hairpin]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:16:52 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 05:43:22 2008''

Revision as of 04:12, 2 July 2008

File:1k43.png

Template:STRUCTURE 1k43

10 Structure Ensemble of the 14-residue peptide RG-KWTY-NG-ITYE-GR (MBH12)

Template:ABSTRACT PUBMED 11782528

About this Structure

Full experimental information is available from OCA.

Reference

Combinatorial approaches: a new tool to search for highly structured beta-hairpin peptides., Pastor MT, Lopez de la Paz M, Lacroix E, Serrano L, Perez-Paya E, Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):614-9. Epub 2002 Jan 8. PMID:11782528

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