1kbd: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,012 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,012 edits
No edit summary
Line 1: Line 1:
[[Image:1kbd.gif|left|200px]]
{{Seed}}
[[Image:1kbd.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1kbd|  PDB=1kbd  |  SCENE=  }}  
{{STRUCTURE_1kbd|  PDB=1kbd  |  SCENE=  }}  


'''SOLUTION STRUCTURE OF A 16 BASE-PAIR DNA RELATED TO THE HIV-1 KAPPA B SITE'''
===SOLUTION STRUCTURE OF A 16 BASE-PAIR DNA RELATED TO THE HIV-1 KAPPA B SITE===




==Overview==
<!--  
1H and 31P NMR spectroscopy have been used together with molecular modelling to determine the fine structure of a non-palindromic 16 bp DNA containing the NF-kappa B binding site. Much emphasis has been placed upon NMR optimization of both two-dimensional 31P NMR techniques to extract structural information defining the phosphodiester backbone conformation and selective homonuclear 2D COSY experiments to determine sugar conformations. NMR data show evidence for a dynamic behaviour of steps flanking the ten base-pairs of the NF-kappa B binding site. A BI-BII equilibrium at these steps is demonstrated and two models for each extreme conformation are proposed in agreement with NMR data. In the refined BII structures, the NF-kappa B binding site exhibits an intrinsic curvature towards the major groove that is magnified by the four flanking steps in the BII conformation. Furthermore, the base-pairs are translated into the major groove. Thus, we present a novel mode of dynamic intrinsic curvature compatible with the DNA curvature observed in the X-ray structure of the p50-DNA complex.
The line below this paragraph, {{ABSTRACT_PUBMED_9636705}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 9636705 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_9636705}}


==About this Structure==
==About this Structure==
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBD OCA].  
Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KBD OCA].  


==Reference==
==Reference==
Line 28: Line 32:
[[Category: Modeling]]
[[Category: Modeling]]
[[Category: Nmr]]
[[Category: Nmr]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:32:03 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:04:45 2008''

Revision as of 07:04, 2 July 2008

File:1kbd.png

Template:STRUCTURE 1kbd

SOLUTION STRUCTURE OF A 16 BASE-PAIR DNA RELATED TO THE HIV-1 KAPPA B SITE

Template:ABSTRACT PUBMED 9636705

About this Structure

Full experimental information is available from OCA.

Reference

Solution structure of a non-palindromic 16 base-pair DNA related to the HIV-1 kappa B site: evidence for BI-BII equilibrium inducing a global dynamic curvature of the duplex., Tisne C, Hantz E, Hartmann B, Delepierre M, J Mol Biol. 1998 May 29;279(1):127-42. PMID:9636705

Page seeded by OCA on Wed Jul 2 10:04:45 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1kbd&oldid=620023"