1kbr: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,615 edits
No edit summary
Line 1: Line 1:
[[Image:1kbr.jpg|left|200px]]
{{Seed}}
[[Image:1kbr.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1kbr|  PDB=1kbr  |  SCENE=  }}  
{{STRUCTURE_1kbr|  PDB=1kbr  |  SCENE=  }}  


'''CRYSTAL STRUCTURE OF UNLIGATED HPPK(R92A) FROM E.COLI AT 1.55 ANGSTROM RESOLUTION'''
===CRYSTAL STRUCTURE OF UNLIGATED HPPK(R92A) FROM E.COLI AT 1.55 ANGSTROM RESOLUTION===




==Overview==
<!--  
6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of the enzyme. At 0.89-A resolution, two distinct conformations are observed for each of the two residues in the crystal structure of the wild-type HPPK in complex with two HP variants, two Mg(2+) ions, and an ATP analogue. Structural information suggests that R92 first binds to the alpha-phosphate group of ATP and then shifts to interact with the beta-phosphate as R82, which initially does not bind to ATP, moves in and binds to alpha-phosphate when the pyrophosphoryl transfer is about to occur. The dynamic roles of R82 and R92 are further elucidated by five more crystal structures of two mutant proteins, R82A and R92A, with and without bound ligands. Two oxidized forms of HP are observed with an occupancy ratio of 0.50:0.50 in the 0.89-A structure. The oxidation of HP has significant impact on its binding to the protein as well as the conformation of nearby residue W89.
The line below this paragraph, {{ABSTRACT_PUBMED_12578370}}, adds the Publication Abstract to the page
(as it appears on PubMed at http://www.pubmed.gov), where 12578370 is the PubMed ID number.
-->
{{ABSTRACT_PUBMED_12578370}}


==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Pyrophosphoryl transfer]]
[[Category: Pyrophosphoryl transfer]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:33:01 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:05:51 2008''

Revision as of 07:05, 2 July 2008

File:1kbr.png

Template:STRUCTURE 1kbr

CRYSTAL STRUCTURE OF UNLIGATED HPPK(R92A) FROM E.COLI AT 1.55 ANGSTROM RESOLUTION

Template:ABSTRACT PUBMED 12578370

About this Structure

1KBR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Dynamic roles of arginine residues 82 and 92 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: crystallographic studies., Blaszczyk J, Li Y, Shi G, Yan H, Ji X, Biochemistry. 2003 Feb 18;42(6):1573-80. PMID:12578370

Page seeded by OCA on Wed Jul 2 10:05:51 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1kbr&oldid=620075"