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[[Image:1kdg.gif|left|200px]]
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[[Image:1kdg.png|left|200px]]


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{{STRUCTURE_1kdg|  PDB=1kdg  |  SCENE=  }}  
{{STRUCTURE_1kdg|  PDB=1kdg  |  SCENE=  }}  


'''Crystal structure of the flavin domain of cellobiose dehydrogenase'''
===Crystal structure of the flavin domain of cellobiose dehydrogenase===




==Overview==
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Cellobiose dehydrogenase (CDH) participates in the degradation of cellulose and lignin. The protein is an extracellular flavocytochrome with a b-type cytochrome domain (CYT(cdh)) connected to a flavodehydrogenase domain (DH(cdh)). DH(cdh) catalyses a two-electron oxidation at the anomeric C1 position of cellobiose to yield cellobiono-1,5-lactone, and the electrons are subsequently transferred from DH(cdh) to an acceptor, either directly or via CYT(cdh). Here, we describe the crystal structure of Phanerochaete chrysosporium DH(cdh) determined at 1.5 A resolution. DH(cdh) belongs to the GMC family of oxidoreductases, which includes glucose oxidase (GOX) and cholesterol oxidase (COX); however, the sequence identity with members of the family is low. The overall fold of DH(cdh) is p-hydroxybenzoate hydroxylase-like and is similar to, but also different from, that of GOX and COX. It is partitioned into an FAD-binding subdomain of alpha/beta type and a substrate-binding subdomain consisting of a seven-stranded beta sheet and six helices. Docking of CYT(cdh) and DH(cdh) suggests that CYT(cdh) covers the active-site entrance in DH(cdh), and that the resulting distance between the cofactors is within acceptable limits for inter-domain electron transfer. Based on docking of the substrate, cellobiose, in the active site of DH(cdh), we propose that the enzyme discriminates against glucose by favouring interaction with the non-reducing end of cellobiose.
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{{ABSTRACT_PUBMED_11786022}}


==About this Structure==
==About this Structure==
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[[Category: Phbh fold]]
[[Category: Phbh fold]]
[[Category: Rossmann fold]]
[[Category: Rossmann fold]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:36:21 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:10:12 2008''

Revision as of 07:10, 2 July 2008

File:1kdg.png

Template:STRUCTURE 1kdg

Crystal structure of the flavin domain of cellobiose dehydrogenase

Template:ABSTRACT PUBMED 11786022

About this Structure

1KDG is a Single protein structure of sequence from Phanerochaete chrysosporium. Full crystallographic information is available from OCA.

Reference

Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase., Hallberg BM, Henriksson G, Pettersson G, Divne C, J Mol Biol. 2002 Jan 18;315(3):421-34. PMID:11786022

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