1kf2: Difference between revisions

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[[Image:1kf2.jpg|left|200px]]
{{Seed}}
[[Image:1kf2.png|left|200px]]


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{{STRUCTURE_1kf2|  PDB=1kf2  |  SCENE=  }}  
{{STRUCTURE_1kf2|  PDB=1kf2  |  SCENE=  }}  


'''Atomic Resolution Structure of RNase A at pH 5.2'''
===Atomic Resolution Structure of RNase A at pH 5.2===




==Overview==
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The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.
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{{ABSTRACT_PUBMED_11856829}}


==About this Structure==
==About this Structure==
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Revision as of 07:14, 2 July 2008

File:1kf2.png

Template:STRUCTURE 1kf2

Atomic Resolution Structure of RNase A at pH 5.2

Template:ABSTRACT PUBMED 11856829

About this Structure

1KF2 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Atomic resolution structures of ribonuclease A at six pH values., Berisio R, Sica F, Lamzin VS, Wilson KS, Zagari A, Mazzarella L, Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):441-50. Epub 2002, Feb 21. PMID:11856829

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