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[[Image:1kfg.gif|left|200px]]
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[[Image:1kfg.png|left|200px]]


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{{STRUCTURE_1kfg|  PDB=1kfg  |  SCENE=  }}  
{{STRUCTURE_1kfg|  PDB=1kfg  |  SCENE=  }}  


'''The X-ray Crystal Structure of Cel9G from Clostridium cellulolyticum complexed with a Thio-Oligosaccharide Inhibitor'''
===The X-ray Crystal Structure of Cel9G from Clostridium cellulolyticum complexed with a Thio-Oligosaccharide Inhibitor===




==Overview==
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Complete cellulose degradation is the first step in the use of biomass as a source of renewable energy. To this end, the engineering of novel cellulase activity, the activity responsible for the hydrolysis of the beta-1,4-glycosidic bonds in cellulose, is a topic of great interest. The high-resolution X-ray crystal structure of a multidomain endoglucanase from Clostridium cellulolyticum has been determined at a 1.6-A resolution. The endoglucanase, Cel9G, is comprised of a family 9 catalytic domain attached to a family III(c) cellulose-binding domain. The two domains together form a flat platform onto which crystalline cellulose is suggested to bind and be fed into the active-site cleft for endolytic hydrolysis. To further dissect the structural basis of cellulose binding and hydrolysis, the structures of Cel9G in the presence of cellobiose, cellotriose, and a DP-10 thio-oligosaccharide inhibitor were resolved at resolutions of 1.7, 1.8, and 1.9 A, respectively.
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{{ABSTRACT_PUBMED_12837787}}


==About this Structure==
==About this Structure==
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[[Category: Thio-oligosaccharide]]
[[Category: Thio-oligosaccharide]]
[[Category: X-ray diffraction]]
[[Category: X-ray diffraction]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:40:53 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:15:44 2008''

Revision as of 07:15, 2 July 2008

File:1kfg.png

Template:STRUCTURE 1kfg

The X-ray Crystal Structure of Cel9G from Clostridium cellulolyticum complexed with a Thio-Oligosaccharide Inhibitor

Template:ABSTRACT PUBMED 12837787

About this Structure

1KFG is a Single protein structure of sequence from Clostridium cellulolyticum. Full crystallographic information is available from OCA.

Reference

X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides., Mandelman D, Belaich A, Belaich JP, Aghajari N, Driguez H, Haser R, J Bacteriol. 2003 Jul;185(14):4127-35. PMID:12837787

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